Optimizing Gel Formulations Using Carbopol 940 and Sodium Alginate Containing Andrographis paniculata Extract for Burn-Wound Healing

JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Pub Date : 2023-12-21 DOI:10.20473/jfiki.v10i32023.300-311

E. F. Apriani, Naisa Kornelia, Annisa Amriani

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Abstract

Background: Sambiloto leaves (Andrographis paniculata (Burm.f.) Nees) contain andrographolide (diterpene lactone), flavonoids, quinic acid, steroids, saponins, alkaloids, and tannins, which act as anti-inflammatory, antioxidant, antibacterial, and wound healing. Objective: This study optimizes the gelling ingredient in Sambiloto extract gel preparations (Andrographis paniculata (Burm.f.) Ness) as a wound healer in male Wistar rats. The gelling agent is an important component that can affect active substance release. Methods: Formula optimization was developed using the Regular Two-Level 22 Factorial Design method in Design-Expert 12 software. This study used 0.5%-1% carbopol 940 and 1%-5% sodium alginate. Carbopol 940 and Sodium Alginate have different characteristics, so they need to be optimized to produce a gel with good characteristics. Results: Physical property evaluation using factorial design revealed the optimal formula at 0.5% carbopol and 5% sodium alginate, with average pH, viscosity, and adhesion values of 5.17 ± 0.04; 2934.452±286.871 cPs; and 194.236±3.684 s. Centrifugation and cycling tests indicated no organoleptic changes, phase separation, or significant changes in pH. ANOVA analysis showed that the gel with 10% Sambiloto leaf extract had similar burn healing activity to the positive control, with a recovery rate of 99.72 ± 0.47% in 20 days. Scabs formed on the 8th day and peeled off on the 12th day. Conclusion: Sambiloto extract gel in the optimum formula has the potential to be developed as a burn wound-healing drug.

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使用含有穿心莲提取物的Carbopol 940和海藻酸钠优化凝胶配方，促进烧伤伤口愈合

背景：穿心莲叶片（穿心莲（Burm.f. Nees））含有穿心莲内酯（二萜内酯）、黄酮类、奎宁酸、类固醇、皂甙、生物碱和单宁酸，具有消炎、抗氧化、抗菌和伤口愈合的作用。研究目的本研究优化了穿心莲提取物凝胶制剂（穿心莲（Burm.f. Ness））中的胶凝成分，将其作为雄性 Wistar 大鼠的伤口愈合剂。胶凝剂是影响活性物质释放的重要成分。方法：使用 Design-Expert 12 软件中的常规两级 22 因式设计法进行配方优化。本研究使用了 0.5%-1% 的 Carbopol 940 和 1%-5% 的海藻酸钠。Carbopol 940 和海藻酸钠具有不同的特性，因此需要对它们进行优化，以生产出具有良好特性的凝胶。结果采用因子设计进行的物理性质评估显示，最佳配方为 0.5% 的 Carbopol 和 5% 的海藻酸钠，平均 pH 值、粘度和粘附性分别为 5.17 ± 0.04、2934.452±286.871 cPs 和 194.236±3.684 s。方差分析显示，含有 10%桑比洛托叶提取物的凝胶具有与阳性对照相似的烧伤愈合活性，20 天内的恢复率为 99.72 ± 0.47%。痂皮在第 8 天形成，第 12 天脱落。结论采用最佳配方的桑比洛托提取物凝胶具有开发烧伤创面愈合药物的潜力。

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JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA

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12 weeks