Genome Sequence and Characterisation of Peribacillus sp. Strain AS_2, a Bacterial Endophyte Isolated from Alectra sessiliflora

IF 2.1 Q3 MICROBIOLOGY

Microbiology Research Pub Date : 2023-12-21 DOI:10.3390/microbiolres15010004

M. P. Maela, M. H. Serepa-Dlamini

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Abstract

Peribacillus sp. AS_2, a leaf endophytic bacterium isolated from the medicinal plant Alectra sessiliflora, was previously identified using the 16S rRNA gene sequence. The draft genome of AS_2 had a 5,482,853 bp draft circular chromosome, 43 contigs, N50 of 360,633 bp and an average G + C% content of 40.5%. Whole genome sequencing and phenotypic analysis showed that AS_2 was Gram-positive, endospore-forming, motile, and rod-shaped and it showed a high sequence similarity with P. frigoritolerans DSM 8801T. Genomic digital DNA–DNA hybridisation (dDDH) between strain AS_2 and Peribacillus frigoritolerans DSM 8801T and P. castrilensis N3T was 84.8% and 79.2%, respectively, and the average nucleotide identity (ANI) of strain AS_2 with P. frigoritolerans DSM 8801T and P. castrilensis N3T was 97.0% and 96.7%, respectively. The antiSMASH software predicted a total of eight secondary metabolite gene clusters comprising non-ribosomal peptide synthetase (NRPS) type koranimine, terpenes, and siderophore clusters. Strain AS_2 also displayed genes involved in endophytic lifestyle and antibiotic resistance gene clusters such as small multidrug resistance antibiotic efflux pumps (qacJs). Using the multilocus sequence analysis (MLSA), together with the phenotypic data and genomic analysis, we demonstrated that strain AS_2 is a subspecies of P. frigoritolerans DSM 8801T. Genome sequencing of Peribacillus sp. AS_2 from medicinal plants provides valuable genomic information and allows us to further explore its biotechnological applications.

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Peribacillus sp.菌株 AS_2 的基因组序列和特征，它是一种从 Alectra sessiliflora 分离出来的内生细菌

先前利用 16S rRNA 基因序列鉴定了从药用植物 Alectra sessiliflora 分离出的叶内生细菌 Peribacillus sp.AS_2 的基因组草案有一个 5,482,853 bp 的环状染色体草案，43 个等位基因，N50 为 360,633 bp，平均 G + C% 含量为 40.5%。全基因组测序和表型分析表明，AS_2呈革兰氏阳性，有内生孢子，能运动，呈杆状，与P. frigoritolerans DSM 8801T的序列高度相似。AS_2菌株与Peribacillus frigoritolerans DSM 8801T和P. castrilensis N3T的基因组数字DNA-DNA杂交（dDDH）分别为84.8%和79.2%，AS_2菌株与P. frigoritolerans DSM 8801T和P. castrilensis N3T的平均核苷酸同一性（ANI）分别为97.0%和96.7%。反SMASH软件共预测了8个次级代谢物基因簇，包括非核糖体肽合成酶（NRPS）型柯拉明、萜类和苷元簇。菌株 AS_2 还显示了参与内生生活方式的基因和抗生素抗性基因簇，如小型多药抗性抗生素外排泵（qacJs）。利用多焦点序列分析（MLSA）、表型数据和基因组分析，我们证明了菌株 AS_2 是 P. frigoritolerans DSM 8801T 的一个亚种。对药用植物中的 Peribacillus sp.

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来源期刊

Microbiology Research MICROBIOLOGY-

CiteScore

1.90

自引率

6.70%

发文量

审稿时长

10 weeks

期刊介绍： Microbiology Research is an international, online-only, open access peer-reviewed journal which publishes original research, review articles, editorials, perspectives, case reports and brief reports to benefit researchers, microbiologists, physicians, veterinarians. Microbiology Research publishes ‘Clinic’ and ‘Research’ papers divided into two different skill and proficiency levels: ‘Junior’ and ‘Professional’. The aim of this four quadrant grid is to encourage younger researchers, physicians and veterinarians to submit their results even if their studies encompass just a limited set of observations or rely on basic statistical approach, yet upholding the customary sound approach of every scientific article.