Occludin is overexpressed in tubo-ovarian high-grade serous carcinoma compared to mesothelioma and is a marker of tumor progression and chemoresistance.

IF 4.2 3区医学 Q2 ONCOLOGY

Clinical & Experimental Metastasis Pub Date : 2024-02-01 Epub Date: 2023-12-23 DOI:10.1007/s10585-023-10251-5

Margarida Varela Dos Santos, Arild Holth, Katharina Bischof, Ben Davidson

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Abstract

The objective of this study was to analyze the expression and prognostic role of the tight junction protein occludin in high-grade serous carcinoma (HGSC). Occludin protein expression by immunohistochemistry was analyzed in 602 HGSC (417 effusions, 185 surgical specimens). Expression in mesothelioma (n = 87; 45 effusions, 42 surgical specimens) was studied for comparative purposes. Occludin protein expression was found in 587/602 (98%) HGSC vs. 40/87 (46%) mesotheliomas and was predominantly limited to < 5% of cells in the latter (p < 0.001). Occludin was additionally overexpressed in HGSC effusions compared to surgical specimens (p < 0.001) and was overexpressed in post-chemotherapy effusions compared to chemo-naive effusions tapped at diagnosis (p = 0.015). Occludin expression in HGSC surgical specimens was associated with poor chemoresponse (p < 0.001) and primary resistance (p = 0.001). Expression in effusions and surgical specimens was unrelated to survival (p > 0.05). In conclusion, occludin expression is higher in HGSC compared to mesothelioma, and this protein is overexpressed in HGSC effusions, possibly reflecting changes in adhesion related to anchorage-independent growth in this microenvironment. Overexpression in post-chemotherapy compared to chemo-naïve effusions suggest a role in disease progression. Occludin expression in surgical specimens may be related to chemoresistance.

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与间皮瘤相比，Occludin 在输卵管卵巢高级别浆液性癌中过度表达，是肿瘤进展和化疗耐药性的标志物。

本研究的目的是分析紧密连接蛋白Occludin在高级别浆液性癌（HGSC）中的表达和预后作用。通过免疫组化方法分析了602例高级别浆液性癌（417例渗出液，185例手术标本）中Occludin蛋白的表达情况。为了进行比较，还研究了间皮瘤（n = 87；45 个渗出液，42 个手术标本）中的表达情况。在 587/602 例（98%）HGSC 与 40/87 例（46%）间皮瘤中发现了 Occludin 蛋白表达，且主要限于 0.05）。总之，Occludin 在 HGSC 中的表达高于间皮瘤，而且这种蛋白在 HGSC 渗出液中过表达，这可能反映了在这种微环境中与锚定依赖性生长相关的粘附性变化。与化疗无效的渗出液相比，化疗后渗出液中这种蛋白的过表达表明它在疾病进展中起着一定的作用。手术标本中 Occludin 的表达可能与化疗耐药性有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical & Experimental Metastasis 医学-肿瘤学

CiteScore

7.80

自引率

5.00%

发文量

审稿时长

12 months

期刊介绍： The Journal''s scope encompasses all aspects of metastasis research, whether laboratory-based, experimental or clinical and therapeutic. It covers such areas as molecular biology, pharmacology, tumor biology, and clinical cancer treatment (with all its subdivisions of surgery, chemotherapy and radio-therapy as well as pathology and epidemiology) insofar as these disciplines are concerned with the Journal''s core subject of metastasis formation, prevention and treatment.