Evaluation of the biocompatibility of fish skin collagen with the mesenchymal stem cells in in vitro cultures.

IF 3.1 4区 医学 Q2 BIOPHYSICS
Kinga Rusinek, Anna Słysz, Tomasz Dębski, Katarzyna Siennicka, Aleksandra Zołocińska, Justyna Miszkiewicz-Jasińska, Anna Aleksandrowicz, Zygmunt Pojda
{"title":"Evaluation of the biocompatibility of fish skin collagen with the mesenchymal stem cells in <i>in vitro</i> cultures.","authors":"Kinga Rusinek, Anna Słysz, Tomasz Dębski, Katarzyna Siennicka, Aleksandra Zołocińska, Justyna Miszkiewicz-Jasińska, Anna Aleksandrowicz, Zygmunt Pojda","doi":"10.1177/22808000231211423","DOIUrl":null,"url":null,"abstract":"<p><p>There are scarce published data suggesting, that collagen extracted from fish skin may be an attractive alternative to mammalian-derived collagen for the <i>in vitro</i> cell cultures. In this study, we investigated proliferation potential and differentiation capability into osteogenic and adipogenic lineages of rat adipose-derived mesenchymal stem cells (rASCs) and human adipose-derived mesenchymal stem cells (hASCs) cultured on collagen extracted from silver carp and African sharptooth catfish skins, compared with commercially available mammalian collagen and collagen-free culture dishes. Our results revealed no significant differences between fish collagen and mammalian collagen in supporting cell viability and proliferation capacity. Fish-derived collagen is a cheap material derived from production waste, does not contain transmissible pathogens of mammalian origin, supports human cell cultures at comparable level to conventional collagen sources, and may be considered as the product of choice for the <i>in vitro</i> cell cultures.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"21 ","pages":"22808000231211423"},"PeriodicalIF":3.1000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Biomaterials & Functional Materials","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1177/22808000231211423","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 0

Abstract

There are scarce published data suggesting, that collagen extracted from fish skin may be an attractive alternative to mammalian-derived collagen for the in vitro cell cultures. In this study, we investigated proliferation potential and differentiation capability into osteogenic and adipogenic lineages of rat adipose-derived mesenchymal stem cells (rASCs) and human adipose-derived mesenchymal stem cells (hASCs) cultured on collagen extracted from silver carp and African sharptooth catfish skins, compared with commercially available mammalian collagen and collagen-free culture dishes. Our results revealed no significant differences between fish collagen and mammalian collagen in supporting cell viability and proliferation capacity. Fish-derived collagen is a cheap material derived from production waste, does not contain transmissible pathogens of mammalian origin, supports human cell cultures at comparable level to conventional collagen sources, and may be considered as the product of choice for the in vitro cell cultures.

评估鱼皮胶原与间充质干细胞在体外培养中的生物相容性。
已发表的数据表明,从鱼皮中提取的胶原蛋白可能是哺乳动物来源胶原蛋白的一种有吸引力的体外细胞培养替代物。在这项研究中,我们研究了大鼠脂肪间充质干细胞(rASCs)和人脂肪间充质干细胞(hASCs)在鲢鱼皮和非洲尖齿鲶鱼皮提取的胶原蛋白上与市售哺乳动物胶原蛋白和无胶原蛋白培养皿相比的增殖潜力以及向成骨系和成脂系分化的能力。结果表明,鱼胶原蛋白与哺乳动物胶原蛋白在支持细胞活力和增殖能力方面没有明显差异。鱼源性胶原蛋白是一种从生产废料中提取的廉价材料,不含哺乳动物来源的可传播病原体,对人类细胞培养的支持水平与传统胶原蛋白来源相当,可视为体外细胞培养的首选产品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信