Characterization of the Chemical Constituents in Radix gentianae by Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-12-20 DOI:10.1093/chromsci/bmad091

Xu Pan, Jiao Guan, Yue Cui, Lele Li, Xiaoying Lin, Bo Feng, Heyun Zhu

引用次数: 0

Abstract

Radix gentianae (RG) is a traditional Chinese medicine used for the treatment of acute and chronic hepatitis in clinic. However, the chemical profile of RG is still unconfirmed, which hindered the progress of pharmacological study and clinical application. In this study, ultra-high performance liquid chromatography together with quadrupole time-of-flight mass spectrometry techniques were employed to separate and characterize the chemical constituents in RG. Under the optimized conditions, a total of 60 compounds were rapidly identified or tentatively characterized. Results indicated that iridoid glucosides, flavonoids, organic acids, amino acids, saccharides and nucleosides were major constituents in RG. It is concluded the established method can help to clarify the substance basis and provide useful information for ascertaining the bioactive constituents and action mechanism of RG.

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超高效液相色谱-四极杆飞行时间质谱法表征龙胆草中的化学成分

龙胆草（RG）是一种传统中药，临床上用于治疗急慢性肝炎。然而，龙胆草的化学成分尚未得到证实，这阻碍了其药理研究和临床应用的进展。本研究采用超高效液相色谱法和四极杆飞行时间质谱法对 RG 中的化学成分进行了分离和表征。在优化的条件下，共对 60 种化合物进行了快速鉴定或初步定性。结果表明，鸢尾苷、黄酮、有机酸、氨基酸、糖类和核苷是 RG 中的主要成分。结论是所建立的方法有助于阐明 RG 的物质基础，并为确定 RG 的生物活性成分和作用机制提供有用信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.