Electrostatics and hydrophobicity in the dynamics of intrinsically disordered proteins

IF 1.8 4区物理与天体物理 Q4 CHEMISTRY, PHYSICAL

The European Physical Journal E Pub Date : 2023-12-21 DOI:10.1140/epje/s10189-023-00383-7

Renee Vancraenenbroeck, Hagen Hofmann

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Abstract

Internal friction is a major contribution to the dynamics of intrinsically disordered proteins (IDPs). Yet, the molecular origin of internal friction has so far been elusive. Here, we investigate whether attractive electrostatic interactions in IDPs modulate internal friction differently than the hydrophobic effect. To this end, we used nanosecond fluorescence correlation spectroscopy (nsFCS) and single-molecule Förster resonance energy transfer (FRET) to quantify the conformation and dynamics of the disordered DNA-binding domains Myc, Max and Mad at different salt concentrations. We find that internal friction effects are stronger when the chain is compacted by electrostatic attractions compared to the hydrophobic effect. Although the effect is moderate, the results show that the heteropolymeric nature of IDPs is reflected in their dynamics.

Graphical abstract

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本质无序蛋白质动力学中的静电和疏水性

内摩擦力是内在无序蛋白（IDPs）动力学的一个主要因素。然而，内摩擦力的分子起源至今仍难以捉摸。在此，我们研究了 IDPs 中的吸引性静电相互作用是否以不同于疏水效应的方式调节内摩擦力。为此，我们使用纳秒荧光相关光谱（nsFCS）和单分子佛斯特共振能量转移（FRET）来量化无序 DNA 结合域 Myc、Max 和 Mad 在不同盐浓度下的构象和动力学。我们发现，与疏水效应相比，当链被静电吸引压实时，内摩擦效应更强。虽然这种效应是温和的，但结果表明 IDPs 的异聚性质反映在其动力学中。

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来源期刊

The European Physical Journal E CHEMISTRY, PHYSICAL-MATERIALS SCIENCE, MULTIDISCIPLINARY

CiteScore

2.60

自引率

5.60%

发文量

审稿时长

3 months

期刊介绍： EPJ E publishes papers describing advances in the understanding of physical aspects of Soft, Liquid and Living Systems. Soft matter is a generic term for a large group of condensed, often heterogeneous systems -- often also called complex fluids -- that display a large response to weak external perturbations and that possess properties governed by slow internal dynamics. Flowing matter refers to all systems that can actually flow, from simple to multiphase liquids, from foams to granular matter. Living matter concerns the new physics that emerges from novel insights into the properties and behaviours of living systems. Furthermore, it aims at developing new concepts and quantitative approaches for the study of biological phenomena. Approaches from soft matter physics and statistical physics play a key role in this research. The journal includes reports of experimental, computational and theoretical studies and appeals to the broad interdisciplinary communities including physics, chemistry, biology, mathematics and materials science.