Patch-seq: Advances and Biological Applications

IF 3.6 4区 医学 Q3 CELL BIOLOGY
Mingting Shao, Wei Zhang, Ye Li, Lei Tang, Zhao-Zhe Hao, Sheng Liu
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引用次数: 0

Abstract

Multimodal analysis of gene-expression patterns, electrophysiological properties, and morphological phenotypes at the single-cell/single-nucleus level has been arduous because of the diversity and complexity of neurons. The emergence of Patch-sequencing (Patch-seq) directly links transcriptomics, morphology, and electrophysiology, taking neuroscience research to a multimodal era. In this review, we summarized the development of Patch-seq and recent applications in the cortex, hippocampus, and other nervous systems. Through generating multimodal cell type atlases, targeting specific cell populations, and correlating transcriptomic data with phenotypic information, Patch-seq has provided new insight into outstanding questions in neuroscience. We highlight the challenges and opportunities of Patch-seq in neuroscience and hope to shed new light on future neuroscience research.

Abstract Image

斑块芯片:进展与生物应用
由于神经元的多样性和复杂性,在单细胞/单核水平对基因表达模式、电生理特性和形态表型进行多模态分析一直是一项艰巨的任务。补丁测序(Patch-sequencing,Patch-seq)的出现将转录组学、形态学和电生理学直接联系起来,将神经科学研究带入了多模态时代。在这篇综述中,我们总结了 Patch-seq 的发展以及最近在大脑皮层、海马和其他神经系统中的应用。通过生成多模态细胞类型图谱、靶向特定细胞群以及将转录组数据与表型信息关联起来,Patch-seq 为神经科学领域的未决问题提供了新的见解。我们强调了 Patch-seq 在神经科学领域的挑战和机遇,希望能为未来的神经科学研究带来新的启示。
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来源期刊
CiteScore
7.70
自引率
0.00%
发文量
137
审稿时长
4-8 weeks
期刊介绍: Cellular and Molecular Neurobiology publishes original research concerned with the analysis of neuronal and brain function at the cellular and subcellular levels. The journal offers timely, peer-reviewed articles that describe anatomic, genetic, physiologic, pharmacologic, and biochemical approaches to the study of neuronal function and the analysis of elementary mechanisms. Studies are presented on isolated mammalian tissues and intact animals, with investigations aimed at the molecular mechanisms or neuronal responses at the level of single cells. Cellular and Molecular Neurobiology also presents studies of the effects of neurons on other organ systems, such as analysis of the electrical or biochemical response to neurotransmitters or neurohormones on smooth muscle or gland cells.
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