Annexin A7 and Its Related Protein Suppressor of Death Domains Regulates Migration and Proliferation of Hca-P Cells.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-11-01 DOI:10.22074/cellj.2023.559724.1108

Shaoqing Wang, Qingyang Bai, Xiuwen Yu, Feng Gao, Yurong Sun, Xianyan Wang

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Abstract

Objective: This study was to investigate whether annexin A7 (AnnexinA7, ANXA7) and its co-related protein tumor cell death domain silencer [suppressor of death domains (SODD)] regulates the migratory phenotype of liver cancer cells.

Materials and methods: In this experimental study, expression of ANXA7 in Hca-P cells, PANXA7 downregulated cells and PANXA7 unrelated sequence cells was detected by real-time quantitative polymerase chain reaction (PCR) at mRNA level and western blotting at protein level. Transwell migration and invasion assays were performed to determine the migratory phenotype.

Results: After inhibition of ANXA7 expression, expression of SODD protein was also significantly decreased (P<0.05). Transwell cell transfer experiments showed that number of tumor cells that penetrated into the cell membrane was significantly reduced after ANXA7 silencing (P<0.05). Transwell cell invasion assay showed that number of tumor cells penetrating into Matrigel was significantly reduced after ANXA7 down-regulation (P<0.05). The CCK8 assay was measured at 0, 24 and 48 hours, and proliferation rate of PANXA7 lower weir cells was slower than that of Hca-P cells and PANXA7 non-related sequence cells (P<0.05).

Conclusion: SODD expression was decreased with the down-regulation of ANXA7. Down-regulating ANXA7 in Hca-P cells decreased proliferation, migration and invasion of tumor cells.

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Annexin A7 及其相关蛋白死亡域抑制因子调控 Hca-P 细胞的迁移和增殖

研究目的本研究旨在探讨Annexin A7（AnnexinA7，ANXA7）及其相关蛋白肿瘤细胞死亡结构域沉默者[suppressor of death domains (SODD)]是否调控肝癌细胞的迁移表型：本实验研究通过实时定量聚合酶链反应（PCR）在mRNA水平和Western印迹在蛋白水平检测ANXA7在Hca-P细胞、PANXA7下调细胞和PANXA7非相关序列细胞中的表达。进行Transwell迁移和侵袭试验以确定迁移表型：结果：抑制 ANXA7 表达后，SODD 蛋白的表达也显著下降（PANXA7 沉默（PANXA7 下调（PPANXA7 lower weir cells was slower than that of Hca-P cells and PANXA7 non-related sequence cells（PConclusion：SODD的表达随着ANXA7的下调而降低。下调Hca-P细胞中的ANXA7可减少肿瘤细胞的增殖、迁移和侵袭。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.