Multi-Lineage Human Endometrial Organoids on Acellular Amniotic Membrane for Endometrium Regeneration.

IF 3.2 4区 医学 Q3 CELL & TISSUE ENGINEERING
Yuhui Xu, Shuyan Cai, Qian Wang, Minzhang Cheng, Xianrui Hui, Emmanuel Enoch Dzakah, Bing Zhao, Xiaojun Chen
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引用次数: 0

Abstract

Asherman's syndrome is an endometrial regeneration disorder resulting from injury to the endometrial basal layer, causing the formation of scar tissue in the uterus and cervix. This usually leads to uterine infertility, menstrual disorders, and placental abnormalities. While stem cell therapy has shown extensive progress in repairing the damaged endometrium and preventing intrauterine adhesion, issues of low engraftment rates, rapid senescence, and the risk of tumorigenesis remain to be resolved for efficient and effective application of this technology in endometrial repair. This study addressed these challenges by developing a co-culture system to generate multi-lineage endometrial organoids (MLEOs) comprising endometrial epithelium organoids (EEOs) and endometrial mesenchymal stem cells (eMSCs). The efficacy of these MLEOs was investigated by seeding them on a biocompatible scaffold, the human acellular amniotic membrane (HAAM), to create a biological graft patch, which was subsequently transplanted into an injury model of the endometrium in rats. The results indicated that the MLEOs on the HAAM patch facilitated endometrial angiogenesis, regeneration, and improved pregnancy outcomes. The MLEOs on the HAAM patch could serve as a promising strategy for treating endometrial injury and preventing Asherman's syndrome.

细胞羊膜上用于子宫内膜再生的多线型人类子宫内膜有机体
阿舍曼综合征是一种子宫内膜再生障碍,是由于子宫内膜基底层受到损伤,导致子宫和宫颈形成瘢痕组织。这通常会导致子宫性不孕、月经紊乱和胎盘异常。虽然干细胞疗法在修复受损子宫内膜和预防宫腔粘连方面取得了广泛进展,但要在子宫内膜修复中高效、有效地应用这项技术,仍需解决移植率低、衰老快和肿瘤发生风险等问题。本研究通过开发一种共培养系统来生成由子宫内膜上皮细胞(EEO)和子宫内膜间充质干细胞(eMSCs)组成的多系子宫内膜器官组织(MLEOs),从而解决了这些难题。研究人员将这些MLEOs播种到生物相容性支架--人无细胞羊膜(HAAM)上,形成生物移植补片,随后将其移植到大鼠子宫内膜损伤模型中,从而研究了这些MLEOs的功效。结果表明,HAAM 补丁上的 MLEOs 促进了子宫内膜血管生成和再生,并改善了妊娠结局。HAAM补片上的MLEOs可作为一种治疗子宫内膜损伤和预防阿什曼氏综合征的有效策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell Transplantation
Cell Transplantation 生物-细胞与组织工程
CiteScore
6.00
自引率
3.00%
发文量
97
审稿时长
6 months
期刊介绍: Cell Transplantation, The Regenerative Medicine Journal is an open access, peer reviewed journal that is published 12 times annually. Cell Transplantation is a multi-disciplinary forum for publication of articles on cell transplantation and its applications to human diseases. Articles focus on a myriad of topics including the physiological, medical, pre-clinical, tissue engineering, stem cell, and device-oriented aspects of the nervous, endocrine, cardiovascular, and endothelial systems, as well as genetically engineered cells. Cell Transplantation also reports on relevant technological advances, clinical studies, and regulatory considerations related to the implantation of cells into the body in order to provide complete coverage of the field.
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