In the footsteps of Heinz Schlesner and Peter Hirsch: Exploring the untapped diversity of the phylum Planctomycetota in isolates from the 1980s to the early 2000s

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nicolai Kallscheuer , Carmen E. Wurzbacher , Ruth A. Schmitz , Christian Jogler
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引用次数: 0

Abstract

Recent sampling and strain isolation campaigns have accelerated research on the bacterial phylum Planctomycetota. The contribution of more than 100 novel isolates to the open collection of currently 123 described planctomycetal species in the last decade benefited greatly from pioneering work conducted in the second half of the last century. One of those pioneers was Heinz Schlesner, who investigated budding and prosthecate bacteria from habitats world-wide during his time at Christian-Albrechts-University Kiel. An outcome of his research was a strain collection with more than 500 isolates belonging to different bacterial phyla, many of which are uncharacterised members of the phylum Planctomycetota. Due to the lack of affordable genome sequencing techniques at the time of their isolation, most of them were characterised based on phenotypic features and DNA-DNA hybridisation experiments. After the retirement of Heinz Schlesner in 2002, the collection was stored for several years and transferred to Jena in 2019. To get a glimpse on the diversity of members from the phylum Planctomycetota in Schlesner’s collection, we here summarised from his records and publications all available information about the collection regarding sampling habitat and phylogeny. Furthermore, we conducted an updated phylogenetic analysis for a representative excerpt of the collection based on the 16S rRNA gene sequence of 59 strains Schlesner deposited in the NCBI database during strain characterisation studies published in the 1980s until the early 2000s. The results support that strains from his collection are still a valuable contribution to expand the cultivated diversity of the understudied phylum Planctomycetota.

追随海因茨-施莱斯纳(Heinz Schlesner)和彼得-赫希(Peter Hirsch)的脚步:从 20 世纪 80 年代至 21 世纪初的分离物中探索扁孢菌门尚未开发的多样性
最近的采样和菌株分离活动加速了对细菌门 Planctomycetota 的研究。上个世纪下半叶进行的开创性工作极大地促进了过去十年中100多个新分离菌株的产生,目前已公开收集了123个已描述的平滑肌菌种。海因茨-施莱斯纳(Heinz Schlesner)是其中的一位先驱,他在基尔基督教阿尔布雷希茨大学(Christian-Albrechts-University Kiel)工作期间,研究了来自世界各地的芽生和原生细菌。他的研究成果之一是收集了属于不同细菌门的 500 多个分离菌株,其中许多是尚未定性的 Planctomycetota 门成员。由于在分离这些菌株时缺乏经济实惠的基因组测序技术,其中大部分菌株的特征是根据表型特征和 DNA-DNA 杂交实验确定的。海因茨-施莱斯纳(Heinz Schlesner)于 2002 年退休后,这些菌种被保存了数年,并于 2019 年转移到耶拿。为了了解施莱斯纳收藏的扁孢菌门成员的多样性,我们在此从他的记录和出版物中总结了有关该收藏的取样栖息地和系统发育的所有可用信息。此外,我们还根据 Schlesner 在 20 世纪 80 年代至 21 世纪初发表的菌株特征研究报告中存入 NCBI 数据库的 59 株菌株的 16S rRNA 基因序列,对其中具有代表性的部分菌株进行了最新的系统发育分析。分析结果表明,他收集的菌株对扩大研究不足的 Planctomycetota 门的栽培多样性仍有重要贡献。
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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