Jiedu Fuzheng decoction improves the proliferation, migration, invasion and EMT of non-small cell lung cancer via the Wnt/β-catenin pathway

IF 2.8 4区生物学 Q3 CELL BIOLOGY

Cell Division Pub Date : 2023-12-16 DOI:10.1186/s13008-023-00105-7

Fang Fang, Xiaowei Jin, Jinming Meng, Jiaqi He, Jiaxiao Wang, Changhong Wang, Sheng Xie, Wei Shi

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引用次数: 0

Abstract

This study aimed to investigate the effect of Jiedu Fuzheng decoction (JFD) in non-small cell lung cancer (NSCLC) and its potential therapeutic mechanism. We prepared JFD-medicated serum from rats and treated NSCLC cells (A549 and NCI-H1650) with 0.5, 1, and 2 mg/mL JFD-medicated serum. CCK-8 and colony formation assays were used to detect cell proliferation. Transwell assays showed that JFD attenuated cell migration and invasion. JFD and SKL2001 (Wnt/β-catenin activator) were simultaneously used to treat NSCLC cells to verify that JFD regulated the biological behavior of NSCLC via Wnt/β-catenin signaling. It was found that 2 mg/mL JFD had the most significant effect on the activity of NSCLC cells. JFD attenuated proliferation and metastasis but increased the proportion of apoptotic cells. At the same time, JFD downregulated N-cadherin, vimentin and β-catenin protein expression in cancer cells. SKL2001 could restore the improvement of JFD on proliferation, metastasis and apoptosis. This study confirmed that JFD suppressed the occurrence and development of NSCLC by regulating Wnt/β-catenin signaling and provided a novel therapeutic scheme for NSCLC.

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解毒扶正煎剂通过 Wnt/β-catenin 通路改善非小细胞肺癌的增殖、迁移、侵袭和 EMT

本研究旨在探讨解毒扶正煎剂（JFD）对非小细胞肺癌（NSCLC）的作用及其潜在的治疗机制。我们制备了大鼠的 JFD 给药血清，并用 0.5、1 和 2 mg/mL JFD 给药血清处理 NSCLC 细胞（A549 和 NCI-H1650）。CCK-8和集落形成试验用于检测细胞增殖。Transwell试验表明，JFD可减少细胞迁移和侵袭。同时使用 JFD 和 SKL2001（Wnt/β-catenin 激活剂）处理 NSCLC 细胞，以验证 JFD 通过 Wnt/β-catenin 信号调节 NSCLC 的生物学行为。研究发现，2 毫克/毫升的 JFD 对 NSCLC 细胞活性的影响最为显著。JFD抑制了细胞的增殖和转移，但增加了凋亡细胞的比例。同时，JFD 下调了癌细胞中 N-cadherin、vimentin 和 β-catenin 蛋白的表达。SKL2001可恢复JFD对细胞增殖、转移和凋亡的改善作用。该研究证实，JFD通过调节Wnt/β-catenin信号传导抑制NSCLC的发生和发展，为NSCLC提供了一种新的治疗方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Division CELL BIOLOGY-

CiteScore

3.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Cell Division is an open access, peer-reviewed journal that encompasses all the molecular aspects of cell cycle control and cancer, cell growth, proliferation, survival, differentiation, signalling, gene transcription, protein synthesis, genome integrity, chromosome stability, centrosome duplication, DNA damage and DNA repair. Cell Division provides an online forum for the cell-cycle community that aims to publish articles on all exciting aspects of cell-cycle research and to bridge the gap between models of cell cycle regulation, development, and cancer biology. This forum is driven by specialized and timely research articles, reviews and commentaries focused on this fast moving field, providing an invaluable tool for cell-cycle biologists. Cell Division publishes articles in areas which includes, but not limited to: DNA replication, cell fate decisions, cell cycle & development Cell proliferation, mitosis, spindle assembly checkpoint, ubiquitin mediated degradation DNA damage & repair Apoptosis & cell death