Genome Survey Sequencing of the Medical Plant Dioscorea futschauensis: Genomic Characteristics Estimation and Genomic Resources Development

IF 0.5 4区 医学
Bizhen Luo, Dong Li, Ke Hu, Min Chen, Xiaoqin Sun, Ruisen Lu, Shujing Wei
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引用次数: 0

Abstract

Dioscorea futschauensis Uline ex R. Knuth, a perennial climbing herb belonging to the Stenophora section of the genus Dioscorea L. (Dioscoreaceae), originates from Southeast China and is highly regarded for its medicinal properties. Despite its medicinal significance, the absence of genomic information has impeded the effective utilization and conservation of this species. Here, we conducted genome survey sequencing of D. futschauensis to unveil its genomic characteristics and identify species-specific genomic markers, including whole plastome sequence, plastome-divergent hotspots, single-copy nuclear genes and polymorphic nuclear SSRs. The genome of D. futschauensis was approximated to be 1392.76 Mb in length, accompanied by a substantial heterozygosity rate of 1.75% and a repeat content of 67.43%. Comparative plastome analysis illuminated the conserved genome structure and gene content within D. futschauensis, and revealed four highly divergent hotspots (atpF intron, psbA-matK, matK -rps16, and rrn16-trnI) that could serve as molecular markers for D. futschauensis and its closely related species. Furthermore, more than 200 single-copy nuclear genes and 78 polymorphic nuclear SSRs were identified within D. futschauensis. Overall, the comprehensive genomic information and abundant genetic resources provided herein will not only facilitate extended research in the realms of population genetics, phylogenetics and conservation biology of D. futschauensis, but also hold considerable value for its cultivation and molecular breeding applications.
药用植物 Dioscorea futschauensis 的基因组调查测序:基因组特征估计和基因组资源开发
山药(Dioscorea futschauensis Uline ex R. Knuth)是山药科山药属(Dioscorea L.)的一种多年生攀援草本植物,原产于中国东南部,因其独特的药用价值而备受重视。尽管其具有重要的药用价值,但基因组信息的缺乏阻碍了该物种的有效利用和保护。本研究通过对福山雪豹进行基因组调查测序,揭示福山雪豹基因组特征,鉴定种特异性基因组标记,包括全质体序列、质体分化热点、单拷贝核基因和多态核ssr。futschauensis基因组长度约为1392.76 Mb,杂合率为1.75%,重复数为67.43%。对比质组分析揭示了该物种保守的基因组结构和基因含量,揭示了4个高度分化的热点(atpF内含子、psbA-matK、matK -rps16和rrn16-trnI),可作为该物种及其近缘种的分子标记。此外,还鉴定出200多个单拷贝核基因和78个多态核ssr。综上所述,本文所提供的全面的基因组信息和丰富的遗传资源,不仅有利于在狐猴种群遗传学、系统遗传学和保护生物学等领域的深入研究,而且对狐猴的培育和分子育种应用具有重要价值。
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来源期刊
Journal of Biobased Materials and Bioenergy
Journal of Biobased Materials and Bioenergy 工程技术-材料科学:生物材料
自引率
0.00%
发文量
60
审稿时长
6 months
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