Assessment of the Toxic Effect of 2-(Chlorodinitromethyl)-4-Methoxy-6-(4-Methylpiperazine-1-yl)-1,3,5-Triazine by Respiratory Activity of Lymphocytes

IF 1.1 Q4 CELL BIOLOGY

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2023-12-10 DOI:10.1134/S1990747823050057

P. V. Iliasov, L. V. Limareva, A. I. Sizova, V. A. Zalomlenkov, A. P. Kuricyna

引用次数: 0

Abstract

A method for evaluation of metabolic characteristics of intact cells based on electrochemical registration of their respiratory activity was used to monitor a reaction of lymphocytes to a potential pharmacological agent, 2-(chlorodinitromethyl)-4-methoxy-6-(4-methylpiperazin-1-yl)-1,3,5-triazine. The method ensured an estimation of cytotoxicity of the test compound and made it possible to determine its minimum toxic concentrations for human lymphocytes. It was shown that the obtained results agree with the data of the reference method, MTT-based cell viability assay.

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通过淋巴细胞的呼吸活动评估 2-(氯二硝基甲基)-4-甲氧基-6-(4-甲基哌嗪-1-基)-1,3,5-三嗪的毒性效应

一种基于呼吸活动电化学登记的完整细胞代谢特征评估方法被用于监测淋巴细胞对潜在药剂 2-（氯二硝基甲基）-4-甲氧基-6-（4-甲基哌嗪-1-基）-1,3,5-三嗪的反应。该方法确保了对试验化合物细胞毒性的估计，并能确定其对人类淋巴细胞的最小毒性浓度。结果表明，所获得的结果与参考方法（基于 MTT 的细胞活力测定法）的数据一致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology CELL BIOLOGY-

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.