The search for inhibitors of somatic embryogenesis in Medicago truncatula

Q3 Agricultural and Biological Sciences

Ecological genetics Pub Date : 2023-12-04 DOI:10.17816/ecogen568377

Zakhar S. Konstantinov, V. Tvorogova, E. A. Potsenkovskaia, Lyudmila A. Lutova

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引用次数: 0

Abstract

The success of protocols for the genetic transformation of legumes is limited by their low ability to regenerate. Plant regeneration can occur both along the path of shoot regeneration and their further rooting, and along the path of somatic embryogenesis (SE). SE is similar to zygotic embryogenesis (ZE). It is a method of asexual reproduction, in which the somatic cell, due to its totipotency, switches on the embryogenesis program. SE and ZE involve common participants in transcriptional, hormonal, and epigenetic control. Like many processes in the plant organism, SE is controlled by the activity of various stimulants or repressors. As a result of transcriptional analysis of embryogenic and non-embryogenic calli ofMedicago truncatulaat different stages of development, putative genes-inhibitors of SE were found. Using the Golden Gate system, we created vectors for agrobacterial transformation to overexpress genes of interest in embryogenic calli and evaluate their effect on SE. Overexpression of two genes encoding the transcription factors from WRKY and Homobox-WOX families had a significant inhibitory effect on SE (the average number of somatic embryos per callus decreased). TheMtCLE16,a previously found SE inhibitor from the CLE peptides group, was edited and heterozygous frameshift mutants were obtained. The impact of loss of its function on the SE capacity remains to be analyzed. This research was supported by the Sirius University of Science and Technology, project PBB-RND-2243.

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寻找美智子（Medicago truncatula）体细胞胚胎发生的抑制剂

豆科植物遗传转化方案的成功受到其低再生能力的限制。植株再生既可以通过茎部再生及其进一步生根途径进行，也可以通过体细胞胚发生途径进行。SE与合子胚胎发生(ZE)相似。这是一种无性繁殖的方法，其中体细胞由于其全能性，开启了胚胎发生程序。SE和ZE涉及转录、激素和表观遗传控制的共同参与者。与植物生物中的许多过程一样，SE受各种刺激物或抑制物的活性控制。通过对不同发育阶段的断头紫花苜蓿胚性愈伤组织和非胚性愈伤组织的转录分析，发现了可能的SE抑制基因。利用Golden Gate系统构建农杆菌转化载体，在胚性愈伤组织中过表达感兴趣的基因，并评价其对SE的影响。编码WRKY和Homobox-WOX家族转录因子的两个基因过表达对SE有显著的抑制作用(每个愈伤组织的体细胞胚数减少)。对先前从CLE肽组中发现的SE抑制剂TheMtCLE16进行编辑，获得杂合移码突变体。其功能丧失对SE容量的影响还有待分析。本研究得到了天狼星科技大学PBB-RND-2243项目的支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ecological genetics Environmental Science-Ecology

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The journal Ecological genetics is an international journal which accepts for consideration original manuscripts that reflect the results of field and experimental studies, and fundamental research of broad conceptual and/or comparative context corresponding to the profile of the Journal. Once a year, the editorial Board reviews and, if necessary, corrects the rules for authors and the journal rubrics.