Catherine C. Bannon, Elizabeth M. Mudge, Erin M. Bertrand
{"title":"Shedding light on cobalamin photodegradation in the ocean","authors":"Catherine C. Bannon, Elizabeth M. Mudge, Erin M. Bertrand","doi":"10.1002/lol2.10371","DOIUrl":null,"url":null,"abstract":"<p>Cobalamin, vitamin B<sub>12</sub>, is an important micronutrient that has been investigated for decades in the marine context because it is required for phytoplankton growth. The biologically active forms (Me-B<sub>12</sub>, Ado-B<sub>12</sub>) and the synthetic form (CN-B<sub>12</sub>) quickly convert to OH-B<sub>12</sub> after light exposure in various aqueous solutions, but puzzlingly have been frequently reported to dominate dissolved cobalamin pools in the sunlit ocean. Here, we document photodegradation timescales for these cobalamin forms in natural seawater using targeted mass spectrometry, providing quantitative evidence that OH-B<sub>12</sub> is expected to be the dominant dissolved form in irradiated seawater. Then, through high-resolution mass spectrometry, we identify four photodegradation products of OH-B<sub>12</sub> which represent potential building blocks microbes could salvage and remodel to satisfy cellular cobalamin requirements. Taken together, these results clarify the impact of light on marine cobalamin dynamics, laying a foundation for a more quantitative understanding of the role of cobalamin in microbial communities and biogeochemical cycles.</p>","PeriodicalId":18128,"journal":{"name":"Limnology and Oceanography Letters","volume":null,"pages":null},"PeriodicalIF":5.1000,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lol2.10371","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Limnology and Oceanography Letters","FirstCategoryId":"93","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/lol2.10371","RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"LIMNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Cobalamin, vitamin B12, is an important micronutrient that has been investigated for decades in the marine context because it is required for phytoplankton growth. The biologically active forms (Me-B12, Ado-B12) and the synthetic form (CN-B12) quickly convert to OH-B12 after light exposure in various aqueous solutions, but puzzlingly have been frequently reported to dominate dissolved cobalamin pools in the sunlit ocean. Here, we document photodegradation timescales for these cobalamin forms in natural seawater using targeted mass spectrometry, providing quantitative evidence that OH-B12 is expected to be the dominant dissolved form in irradiated seawater. Then, through high-resolution mass spectrometry, we identify four photodegradation products of OH-B12 which represent potential building blocks microbes could salvage and remodel to satisfy cellular cobalamin requirements. Taken together, these results clarify the impact of light on marine cobalamin dynamics, laying a foundation for a more quantitative understanding of the role of cobalamin in microbial communities and biogeochemical cycles.
期刊介绍:
Limnology and Oceanography Letters (LO-Letters) serves as a platform for communicating the latest innovative and trend-setting research in the aquatic sciences. Manuscripts submitted to LO-Letters are expected to present high-impact, cutting-edge results, discoveries, or conceptual developments across all areas of limnology and oceanography, including their integration. Selection criteria for manuscripts include their broad relevance to the field, strong empirical and conceptual foundations, succinct and elegant conclusions, and potential to advance knowledge in aquatic sciences.