Genetic structure of two endangered shrubs in Central Asia and northwestern China and the implications for conservation

Abstract

Helianthemum songaricum and H. ordosicum are two shrubs disjunctively distributed in Central Asia and northwestern China. For conservation purposes, we used genotyping by sequencing (GBS) to investigate the genetic diversity and genetic structure of their populations. Based on the GBS data, H. songaricum populations showed considerable genetic differences from H. ordosicum populations. Helianthemum songaricum populations also showed a stronger population genetic structure within the isolated valleys in the Tianshan Mountains than was seen in previous studies. We speculated that the two species may represent two cotemporal migration events between the Middle East and Central Asia/Ordos Plateau since the early Pliocene. Due to the intense uplift of the Tianshan Mountains in the late Pliocene, populations of H. songaricum were isolated in different valleys. Subsequently, the cold glacial climate during the Pleistocene intensified the in situ intraspecific differentiation of the species. Based on our single nucleotide polymorphism data, we propose habitat damage by anthropological activities, where the two species inhabit should be urgently prohibited by executive management. In addition, a nursery for germplasm resources should be urgently set up to culture adequate progenies for the purpose of expanding the populations.