Improving pesticide residue detection: Immobilized enzyme microreactor embedded in microfluidic paper-based analytical devices

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2023-12-09 DOI:10.1016/j.foodchem.2023.138179

Jian Zhang , Yibing Li , Ting Zhang , Zhihong Zheng , Hui Jing , Chunye Liu

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Abstract

Orientationally immobilized enzyme microreactors (OIMERs), embedded in microfluidic paper-based analytical devices (μPADs) were developed for improved detection of pesticide residues in food. Acetylcholinesterase (AChE) was orientationally immobilized on the reusable Part I of the μPADs, using the specific affinity binding of concanavalin A (Con A) to a glycosyl group on AChE. Using the disposable Part II, facile colorimetric quantification was performed with a smartphone and software, or qualitative detection by a naked-eye visual test. The AChE immobilized in OIMERs not only had improved activity and stability, but also high sensitivity, with a limit of detection as low as (0.007 ± 0.003) μg/mL. The method was used to detect pesticides residues in real vegetable samples; the recovery (88.6–102.7%) showed high reliability for pesticide residues detection in foods. A molecular docking study and an enzyme kinetic analysis were conducted to characterize the mechanism of action of the OIMERs.

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改进农药残留检测：嵌入微流控纸质分析装置的固定化酶微反应器

为改进食品中农药残留的检测，开发了定向固定酶微反应器（OIMER），并将其嵌入微流控纸质分析装置（μPADs）中。利用乙酰胆碱酯酶（AChE）与 AChE 上糖基的特异性亲和力，将乙酰胆碱酯酶定向固定在 μPADs 的可重复使用部分 I 上。使用一次性第二部分，可通过智能手机和软件进行简便的比色定量，或通过肉眼目测进行定性检测。固定在 OIMERs 中的 AChE 不仅具有更高的活性和稳定性，而且灵敏度高，检测限低至 (0.007 ± 0.003) μg/mL。该方法被用于检测实际蔬菜样品中的农药残留，回收率（88.6-102.7%）显示了其在食品中农药残留检测方面的高可靠性。为确定 OIMERs 的作用机制，进行了分子对接研究和酶动力学分析。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.