Taxonomic status of intraspecific germplasm resources of Vaccinium uliginosum based on chloroplast matK gene and SRAP molecular markers

IF 1.5 4区农林科学 Q3 PLANT SCIENCES

Journal of Berry Research Pub Date : 2022-05-24 DOI:10.3233/jbr-211559

Jia-Zhuo Chen, Tong Li, Xin Tian, Yu-Hang Zheng, Zhen-Ling Jin, Hou-Nan Cao, Cheng-Wen Zong

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引用次数: 0

Abstract

BACKGROUND:Abundant germplasm resources of Vaccinium uliginosum are present in Changbai Mountain in China, which have considerable research and development value. They include the naturally distributed alpine V. uliginosum, the white-fruited V. uliginosum variety, and various fruit shapes dominatedby ellipses. However, the relevant research is limited, and the taxonomic status of various specific germplasm resources has not been addressed. OBJECTIVE:The genetic relationship and taxonomic status of four species of V. uliginosum germplasm resources in Changbai Mountain were investigated. METHODS:The chloroplast matK gene and SRAP molecular markers of 94 V. uliginosum samples were analyzed. RESULTS:According to the analysis of chloroplast matK gene, all four types were divided into three branches. The results showed low variation among natural samples of V. uliginosum in China and a relationship between haplotype distribution and geographical distribution. The results of the SRAP molecular marker analysis divided the V. uliginosum population into five branches. The multiple individuals of the same trait were clustered together, and different partial samples were clustered together. CONCLUSIONS:Diploid alpine V. uliginosum and tetraploid V. uliginosum were identified as two subspecies of V. uliginosum. The elliptical fruit shapewas the variant of tetraploid round blue fruit V. uliginosum, and the white-fruited V. uliginosum variety is a variant.

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基于叶绿体matK基因和SRAP分子标记的牛蒡种内种质资源分类现状

背景:中国长白山地区存在着丰富的牛痘种质资源，具有相当的研究开发价值。它们包括自然分布于高山的乌里金莲、白果乌里金莲以及以椭圆形为主的各种果实形状。然而，相关研究有限，各种特定种质资源的分类地位尚未得到解决。目的:调查长白山4种紫茎草种质资源的亲缘关系和分类地位。方法:对94份紫茎草样品的叶绿体matK基因和SRAP分子标记进行分析。结果:根据叶绿体matK基因分析，4种类型均可分为3个分支。结果表明，中国天然样品间的变异较小，单倍型分布与地理分布有一定的关系。SRAP分子标记分析结果表明，该种群可分为5个支系。同一性状的多个个体聚类，不同部分样本聚类。结论:鉴定出二倍体和四倍体是高山紫茎花的两个亚种。椭圆型果实为四倍体圆形蓝色果实的变种，白色果实为四倍体圆形蓝色果实的变种。

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来源期刊

Journal of Berry Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

3.50

自引率

11.80%

发文量

期刊介绍： The main objective of the Journal of Berry Research is to improve the knowledge about quality and production of berries to benefit health of the consumers and maintain profitable production using sustainable systems. The objective will be achieved by focusing on four main areas of research and development: From genetics to variety evaluation Nursery production systems and plant quality control Plant physiology, biochemistry and molecular biology, as well as cultural management Health for the consumer: components and factors affecting berries'' nutritional value Specifically, the journal will cover berries (strawberry, raspberry, blackberry, blueberry, cranberry currants, etc.), as well as grapes and small soft fruit in general (e.g., kiwi fruit). It will publish research results covering all areas of plant breeding, including plant genetics, genomics, functional genomics, proteomics and metabolomics, plant physiology, plant pathology and plant development, as well as results dealing with the chemistry and biochemistry of bioactive compounds contained in such fruits and their possible role in human health. Contributions detailing possible pharmacological, medical or therapeutic use or dietary significance will be welcomed in addition to studies regarding biosafety issues of genetically modified plants.