COVID-19: detection methods in post-mortem samples.

IF 4.4 Q1 PATHOLOGY
PATHOLOGICA Pub Date : 2023-10-01 DOI:10.32074/1591-951X-933
Ilaria Tedesco, Federica Zito Marino, Andrea Ronchi, Amaro Nunes Duarte Neto, Marisa Dolhnikoff, Maurizio Municinò, Carlo Pietro Campobasso, Giuseppe Pannone, Renato Franco
{"title":"COVID-19: detection methods in post-mortem samples.","authors":"Ilaria Tedesco, Federica Zito Marino, Andrea Ronchi, Amaro Nunes Duarte Neto, Marisa Dolhnikoff, Maurizio Municinò, Carlo Pietro Campobasso, Giuseppe Pannone, Renato Franco","doi":"10.32074/1591-951X-933","DOIUrl":null,"url":null,"abstract":"<p><p>COVID-19 identification is routinely performed on fresh samples, such as nasopharyngeal and oropharyngeal swabs, even if, the detection of the virus in formalin-fixed paraffin-embedded (FFPE) autopsy tissues could help to underlie mechanisms of the pathogenesis that are not well understood.</p><p><p>The gold standard for COVID-19 detection in FFPE samples remains the qRT-PCR as in swab samples, contextually other methods have been developed, including immunohistochemistry (IHC), and in situ hybridization (ISH). In this manuscript, we summarize the main data regarding the methods of COVID-19 detection in pulmonary and extra-pulmonary post-mortem samples, and especially the sensitivity and specificity of these assays will be discussed.</p>","PeriodicalId":45893,"journal":{"name":"PATHOLOGICA","volume":"115 5","pages":"263-274"},"PeriodicalIF":4.4000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10699333/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PATHOLOGICA","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32074/1591-951X-933","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PATHOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

COVID-19 identification is routinely performed on fresh samples, such as nasopharyngeal and oropharyngeal swabs, even if, the detection of the virus in formalin-fixed paraffin-embedded (FFPE) autopsy tissues could help to underlie mechanisms of the pathogenesis that are not well understood.

The gold standard for COVID-19 detection in FFPE samples remains the qRT-PCR as in swab samples, contextually other methods have been developed, including immunohistochemistry (IHC), and in situ hybridization (ISH). In this manuscript, we summarize the main data regarding the methods of COVID-19 detection in pulmonary and extra-pulmonary post-mortem samples, and especially the sensitivity and specificity of these assays will be discussed.

COVID-19:尸体样本中的检测方法。
尽管在福尔马林固定石蜡包埋(FFPE)尸检组织中检测 COVID-19 有助于揭示尚不十分清楚的发病机制,但对新鲜样本(如鼻咽和口咽拭子)进行 COVID-19 鉴定仍是常规做法。在 FFPE 样本中检测 COVID-19 的金标准仍然是 qRT-PCR,而在拭子样本中则采用其他方法,包括免疫组化(IHC)和原位杂交(ISH)。在本手稿中,我们将总结有关肺部和肺外尸检样本中 COVID-19 检测方法的主要数据,并特别讨论这些检测方法的灵敏度和特异性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
PATHOLOGICA
PATHOLOGICA PATHOLOGY-
CiteScore
5.90
自引率
5.70%
发文量
108
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信