The presence of glutathione peroxidase 8 (GPx8) in rat male genital organs.

IF 1.5 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Jozef Mihalik, Peter Solar, Kristina Curgali, Martin Svana, Andrea Krehelova, Marko Vrzgula, Viera Almasiova, Ingrid Hodorova
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Abstract

Objectives: The aim of our work was to determine the presence of GPx8, the latest discovered member of glutathione peroxidase family, in rat male genital organs.

Background: The oxidative stress is considered as one of the most important causalities of male infertility. To defend itself, the organism comprises many different antioxidants.

Methods: We assessed the GPx8 presence in tissues of genital organs from adult rat Sprague-Dawley males by mRNA expression, Western Blot analysis, and immunohistochemistry.

Results: The highest mRNA and protein levels were detected in the testis, followed by seminal vesicle. Within testis the enzyme was observed predominantly in the Leydig and Sertoli cells, residual and Hermes bodies. In other organs, such as epididymis, seminal vesicle and prostate gland, the GPx8 was seen in the cytoplasm of epithelial cells. The enzyme was also observed in the muscular layer of hollow organs, in blood plasma and extracellular matrix.

Conclusions: The antioxidant enzyme GPx8 was detected in all examined male genital organs. The fact, that the enzyme was released into lumen of genital organs probably means, that GPx8 is also a component of the semen. To our knowledge, this is the first paper describing GPx8 presence in male genital organs of mammals (Fig. 8, Ref. 63).

谷胱甘肽过氧化物酶8 (GPx8)在大鼠雄性生殖器官中的存在。
目的:测定最新发现的谷胱甘肽过氧化物酶家族成员GPx8在大鼠雄性生殖器官中的存在。背景:氧化应激被认为是男性不育最重要的病因之一。为了保护自己,生物体含有许多不同的抗氧化剂。方法:采用mRNA表达、Western Blot、免疫组化等方法检测GPx8在成年雄性sd大鼠生殖器官组织中的表达。结果:mRNA和蛋白水平以睾丸最高,精囊次之。在睾丸内,该酶主要存在于睾丸间质和支持细胞、残体和赫尔姆斯小体中。在其他器官,如附睾、精囊和前列腺,在上皮细胞的细胞质中可见GPx8。在中空器官的肌肉层、血浆和细胞外基质中也观察到该酶。结论:所有男性生殖器官均检测到抗氧化酶GPx8。事实上,这种酶被释放到生殖器官的腔内可能意味着,GPx8也是精液的一种成分。据我们所知,这是第一篇描述GPx8在哺乳动物雄性生殖器官中存在的论文(图8,参考文献63)。
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来源期刊
CiteScore
2.60
自引率
0.00%
发文量
185
审稿时长
3-8 weeks
期刊介绍: The international biomedical journal - Bratislava Medical Journal – Bratislavske lekarske listy (Bratisl Lek Listy/Bratisl Med J) publishes peer-reviewed articles on all aspects of biomedical sciences, including experimental investigations with clear clinical relevance, original clinical studies and review articles.
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