STAM-binding Protein-like 1 Promotes Growth and Migration of Colorectal Cancer by NF-κB Pathway.

IF 1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Protein and Peptide Letters Pub Date : 2023-01-01 DOI:10.2174/0109298665272785231103104118

Xinghua Zhou, Yue Cheng, Jian Kang, Gang Mao

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引用次数: 0

Abstract

Background: STAM-binding protein-like 1 (STAMBPL1) functions as a deubiquitinase to cleave Lys63 ubiquitin linkage, and is associated with cancer dissemination and progression. The role of STAMBPL1 in colorectal cancer (CRC) remains unclear.

Methods: STAMBPL1 expression was determined by western blot and qRT-PCR. Cell proliferation was detected by colony formation and MTT assays, and apoptosis was assessed by flow cytometry. The metastasis was evaluated by transwell and wound healing assays. An animal xenograft experiment was used to investigate the effect of STAMBPL1 on tumor growth.

Results: The expression of STAMBPL1 was elevated in CRC cells. Knockdown of STAMBPL1 reduced cell viability of CRC and suppressed the proliferation, invasion, and migration. Apoptosis of CRC was induced by silence of STAMBPL1. Tumor growth of CRC was also suppressed by the silence of STAMBPL1. Knockdown of STAMBPL1 increased IκB and decreased phosphorylation of IκB to reduce p65 phosphorylation.

Conclusion: Knockdown of STAMBPL1 inhibited cell growth and metastasis of CRC through inactivation of the NF-κB pathway.

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stamm结合蛋白样1通过NF-κB途径促进结直肠癌的生长和迁移

背景:STAMBPL1 (STAMBPL1)作为去泛素酶裂解Lys63泛素连锁，与癌症的传播和进展有关。STAMBPL1在结直肠癌(CRC)中的作用尚不清楚。方法:采用western blot和qRT-PCR检测STAMBPL1的表达。用菌落形成法和MTT法检测细胞增殖，用流式细胞术检测细胞凋亡。通过transwell和伤口愈合试验评估转移情况。采用动物异种移植实验研究STAMBPL1对肿瘤生长的影响。结果:STAMBPL1在结直肠癌细胞中表达升高。STAMBPL1基因敲低可降低结直肠癌细胞活力，抑制增殖、侵袭和迁移。STAMBPL1沉默可诱导结直肠癌细胞凋亡。STAMBPL1的沉默也能抑制结直肠癌的肿瘤生长。STAMBPL1的下调会增加i - κ b，降低i - κ b磷酸化，从而降低p65的磷酸化。结论:STAMBPL1的下调通过NF-κB通路失活抑制结直肠癌细胞的生长和转移。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Protein and Peptide Letters 生物-生化与分子生物学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Protein & Peptide Letters publishes letters, original research papers, mini-reviews and guest edited issues in all important aspects of protein and peptide research, including structural studies, advances in recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, and drug design. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallization and preliminary structure determination of biologically important proteins are considered only if they include significant new approaches or deal with proteins of immediate importance, and preliminary structure determinations of biologically important proteins. Purely theoretical/review papers should provide new insight into the principles of protein/peptide structure and function. Manuscripts describing computational work should include some experimental data to provide confirmation of the results of calculations. Protein & Peptide Letters focuses on: Structure Studies Advances in Recombinant Expression Drug Design Chemical Synthesis Function Pharmacology Enzymology Conformational Analysis Immunology Biotechnology Protein Engineering Protein Folding Sequencing Molecular Recognition Purification and Analysis