{"title":"Melatonin alleviates the toxic effects of lead by modulating the antioxidative properties in Plantago ovata seedlings","authors":"Shreosi Chakraborty, Sarmistha Sen Raychaudhuri","doi":"10.1007/s11738-023-03626-4","DOIUrl":null,"url":null,"abstract":"<div><p>Our present study focuses on the effect of exogenous application of melatonin on growth, and improvement in oxidative stress in a medicinal herb, isabgol (<i>Plantago ovata</i>). The pretreatment of <i>P. ovata</i> seeds with 25 and 50 μM melatonin under exposure to different concentrations of Pb, i.e., 50, 100, 200 and 500 μM showed variation in dose-dependent responses. Our findings reveal that the melatonin alleviated Pb stress, as reflected in the improvement of plant height and biomass. The photosynthetic pigments and total protein content are also ameliorated in the presence of Pb stress when treated with melatonin. Melatonin, a potent antioxidant, increases the total antioxidant activity, flavonoid contents and DPPH radical scavenging activity while reducing the stress markers such as H<sub>2</sub>O<sub>2</sub> content and MDA content. Pb competitively enters into Ca<sup>2+</sup>-gated channels as one of the mechanisms of Pb uptake in plants. Melatonin was found to reduce Pb accumulation while modulating endogenous Ca content in <i>P. ovata</i>. Furthermore, melatonin significantly increased the activities of antioxidant enzymes such as peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and ascorbate oxidase (AO) in Pb-stressed samples. The heatmap and principal component analysis also validated the beneficial effect of melatonin in improving plant growth parameters.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":null,"pages":null},"PeriodicalIF":2.4000,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Physiologiae Plantarum","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s11738-023-03626-4","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Our present study focuses on the effect of exogenous application of melatonin on growth, and improvement in oxidative stress in a medicinal herb, isabgol (Plantago ovata). The pretreatment of P. ovata seeds with 25 and 50 μM melatonin under exposure to different concentrations of Pb, i.e., 50, 100, 200 and 500 μM showed variation in dose-dependent responses. Our findings reveal that the melatonin alleviated Pb stress, as reflected in the improvement of plant height and biomass. The photosynthetic pigments and total protein content are also ameliorated in the presence of Pb stress when treated with melatonin. Melatonin, a potent antioxidant, increases the total antioxidant activity, flavonoid contents and DPPH radical scavenging activity while reducing the stress markers such as H2O2 content and MDA content. Pb competitively enters into Ca2+-gated channels as one of the mechanisms of Pb uptake in plants. Melatonin was found to reduce Pb accumulation while modulating endogenous Ca content in P. ovata. Furthermore, melatonin significantly increased the activities of antioxidant enzymes such as peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and ascorbate oxidase (AO) in Pb-stressed samples. The heatmap and principal component analysis also validated the beneficial effect of melatonin in improving plant growth parameters.
期刊介绍:
Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry.
The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.