[Evaluation of the cytotoxicity of two capping materials: in vitro cell culture study].

M Soudry, N Pourreau-Schneider, C Salmon, J C Franquin
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Abstract

Evaluation of the biocompatibility of a crown reconstruction material in vivo on human teeth by histologic observation of pulpal reactions is a long lasting and expensive procedure. Before to start it, a first examination can be done by testing the material on cell cultures. If the used culture cells contain antibiotics, it is necessary to combine these tests with a bacteriologic examination of the tested material. Two calcium hydroxide containing materials for dentin-pulp capping, were submitted to cytotoxicity tests using human pulpal fibroblasts cultures. The cytotoxicity of one over the two materials could be related to its lability and bacterial contamination.

[两种封盖材料细胞毒性评价:体外细胞培养研究]。
通过对牙髓反应的组织学观察来评价冠重建材料在人牙体上的生物相容性是一项耗时且昂贵的过程。在开始之前,可以通过在细胞培养物上测试材料来进行第一次检查。如果使用的培养细胞含有抗生素,则有必要将这些测试与被测试材料的细菌学检查结合起来。两种含氢氧化钙的材料用于牙本质-牙髓盖层,提交了人类牙髓成纤维细胞培养的细胞毒性试验。两种材料中的一种的细胞毒性可能与其不稳定性和细菌污染有关。
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