In vitro induction of a diphtheria toxoid specific antibody response in human peripheral blood lymphocytes cultivated in the presence of diphtheria toxoid

Journal of biological standardization Pub Date : 1989-01-01 DOI:10.1016/S0092-1157(89)80002-2

H.G. Loggen , J.G. Kreeftenberg

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引用次数: 2

Abstract

In comparison with the presently used potency test for diphtheria vaccine, in vitro examination of the immunogenicity of the vaccine would have great advantages. For this reason in vitro induction of diphtheria toxoid specific antibody synthesis in human peripheral blood lymphocytes cultivated in the presence of diphtheria toxoid was investigated. The results showed that a dose dependent synthesis of diphtheria antibody was induced by adsorbed diphtheria toxoid and combined vaccines containing the diphtheria toxoid component. Plain diphtheria toxoid appeared to be less immunogenic in comparison with adsorbed toxoid. There is some indication that the pertussis component had a stimulating effect on the diphtheria antibody synthesis. In conclusion, these results are promising for in vitro examination of the immunogenicity of diphtheria vaccines. The model will be validated for the routine control of diphtheria vaccine.

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在白喉类毒素存在下培养的人外周血淋巴细胞体外诱导白喉类毒素特异性抗体反应

与目前使用的白喉疫苗效价试验相比，体外检测白喉疫苗的免疫原性具有很大的优势。为此，研究了在白喉类毒素存在下培养的人外周血淋巴细胞体外诱导白喉类毒素特异性抗体合成的方法。结果表明，吸附白喉类毒素和含白喉类毒素成分的联合疫苗可诱导白喉抗体的剂量依赖性合成。与吸附的类毒素相比，普通白喉类毒素的免疫原性较低。有迹象表明百日咳成分对白喉抗体合成有刺激作用。总之，这些结果为白喉疫苗免疫原性的体外检测提供了良好的前景。该模型将用于白喉疫苗的常规控制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of biological standardization

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