Study on the Protective Effect of Platelet-Rich Plasma Combined with Injection of Pain Points Around Knee Joint on Knee Osteoarthritis and its Molecular Mechanism.

IF 1.9 4区医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE

Alternative therapies in health and medicine Pub Date : 2024-08-01

Xuelian Zhao, Qianxi Zhang, Zan Bu

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引用次数: 0

Abstract

Objective: Previous studies showed the PRP have the therapeutic effects for KOA, but the more detail roles remained unclear and therefore this study was carried on to explore the deeper mechanisms for PRP.

Methods: NRS and WOMAC scores were used to evaluate the clinical efficacy before surgery, 1 month and 6 months after surgery, and the postoperative joint structure changes (n = 24). Examination of pathology of the femoral condyle plate in rats using HE staining (n = 6); Angiogenesis experiments were used to investigate the effect of different groups of cell culture supernatants on the tubular structure formation capacity of HUVECs (n = 3). Observe the proliferation of chondrocytes using clonal formation experiments (n = 3). Western blot was used to analyze the PI3K/AKT signaling pathway and the expression of exosome-secretion-related proteins (n = 3).

Results: In clinical studies, PRP can reduce patients' NRS and WOMAC scores and alleviate the progression of knee arthritis; In rat experiments, PRP reduced damage from knee arthritis and lowered Mankin's score. PRP improves tubular formation of HUVECs and the proliferation capacity of chondrocytes. Compared with the blank control group, the protein expression levels of PI3K, AKT, mTOR, P27, and cyclinD1 in the PRP group were increased. Compared with the PRP group, the protein expression levels of PI3K, AKT, mTOR, P27, and cyclinD1 in the PRP+Axitinib group supplemented with VEGFRs inhibitor and PI3K inhibitor group were significantly decreased. The effect of the LY294002 +PRP group was better than that of the above groups. Macrophage-derived exosomes activate HIF-1a and COX-2 in endothelial cells to promote chondrocyte repair of KOA.

Conclusion: PRP can promote chondrocytes proliferation and repair by activating the PI3K/AKT signaling pathway, thereby exerting anti-OA effects. It provides new targets and methods for the clinical treatment of OA.

本刊更多论文

富血小板血浆联合膝关节周围痛点注射对膝关节骨性关节炎的保护作用及其分子机制的研究。

目的:以往研究表明PRP对KOA有治疗作用，但其具体作用尚不清楚，本研究进一步探讨PRP的深层作用机制。方法:采用NRS评分和WOMAC评分对患者术前、术后1个月、6个月的临床疗效及术后关节结构变化进行评价(n = 24)。大鼠股骨髁钢板HE染色病理检查(n = 6);血管生成实验观察不同组细胞培养上清对HUVECs小管结构形成能力的影响(n = 3)，克隆形成实验观察软骨细胞增殖情况(n = 3)， Western blot分析PI3K/AKT信号通路及外泌体分泌相关蛋白表达情况(n = 3)。在临床研究中，PRP可以降低患者的NRS和WOMAC评分，缓解膝关节关节炎的进展;在大鼠实验中，PRP减轻了膝关节关节炎的损伤，降低了曼金的评分。PRP促进huvec的小管形成和软骨细胞的增殖能力。与空白对照组比较，PRP组PI3K、AKT、mTOR、P27、cyclinD1蛋白表达水平升高。与PRP组相比，PRP+阿西替尼加VEGFRs抑制剂组和PI3K抑制剂组的PI3K、AKT、mTOR、P27、cyclinD1蛋白表达水平均显著降低。LY294002 +PRP组效果优于上述各组。巨噬细胞来源的外泌体激活内皮细胞中的HIF-1a和COX-2，促进KOA的软骨细胞修复。结论:PRP通过激活PI3K/AKT信号通路促进软骨细胞增殖和修复，从而发挥抗oa作用。为骨关节炎的临床治疗提供了新的靶点和方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Alternative therapies in health and medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

0.90

自引率

0.00%

发文量

219

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