Experimental Study of Skin Contraction Induced by Bipolar Radiofrequency.

IF 1.9 4区医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE

Alternative therapies in health and medicine Pub Date : 2024-08-01

Jia Liu, Zhijie Zhao, Jun Zhang, Zhibing Ma, Haonan Peng, Jinlong Huang

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引用次数: 0

Abstract

Background: Facial skin relaxation has become an important part in solving the problem of facial rejuvenation. Minimally invasive or noninvasive skin-tightening procedures have become a trend for facial rejuvenation. Bipolar radiofrequency (RF) is a new option for treating skin relaxation and is more effective than noninvasive surgery without surgical incision.

Objective: To explore the effect of different bipolar RF powers on the area of the original box, changes of skin and subcutaneous tissue thickness and numbers of fibroblasts in rabbits.

Design: The research team performed an animal study.

Setting: This study took place in Affiliated Hospital of Nanjing University of Chinese Medicine.

Participants: Eighteen common-grade adult New Zealand rabbits (female, 2.5-3.0 kg).

Methods: Bipolar radiofrequency therapy was given to a girl rabbit on the left side of the treatment area. Standard HE and Masson staining were performed to assess the pathological changes, area of the original box and the number of fibroblasts in skin and subcutaneous tissues.

Outcome measures: (1) The area of the original box, changes of skin and subcutaneous tissue thickness, and numbers of fibroblasts under different bipolar RF temperatures or under different bipolar RF powers immediately after surgery, 1 month after surgery and 3 months after surgery were observed. (2) Standard HE and Masson staining results.

Results: Under the condition of certain instrument power, at 36de 38d and 40nd the area of the original box shrank to different degrees immediately after surgery (16.54±0.37, 17.78±0.03, 17.19±0.01), 1 month after surgery (16.59±0.31, 17.82±0.01, 18.34±0.30) and 3 months after surgery (16.89±0.12, 18.16±0.14, 19.23±0.32) compared with that before surgery (P < .05). Under specific temperature conditions, at 16 W, 18 W, 20 W, and 22 W, the area of the original box shrank to different degrees immediately after surgery (16.40±0.49, 15.55±0.57, 17.54±0.12, 16.19±0.27), 1 month after surgery (16.88±0.12, 17.46±0.02, 18.05±0.35, 19.41±0.08) and 3 months after surgery (19.09±1.01, 18.30±0.69, 20.00±0.29, 21.20±0.90) compared with that before surgery (P < .05). When the power was fixed, the thickness of skin and subcutaneous tissue decreased immediately after surgery (6.7, 6.8, 7), 1 month after surgery (6, 6.1, 6.3) and 3 months after surgery (6.4, 6.5, 6.2) at different temperatures (P < .05). When the temperature was fixed, the thickness of skin and subcutaneous tissue decreased immediately after surgery (6.1, 6.08, 6.03), 1 month after surgery (6.2, 6.15, 6.13), and 3 months after surgery (6.2, 6.23, 6.03) under different powers (P < .05). Under the condition of certain instrument power, at 36de 38d and 40n, the number of fibroblasts increased to different degrees immediately after surgery (26.54±2.37, 30.78±3.03, 37.19±4.01), 1 month after surgery (28.59±2.31, 34.82±3.01, 40.34±4.30), and 3 months after surgery (30.89±0.12, 38.16±0.14, 42.23±0.32) compared with that before surgery, and all were statistically significant (P < .05). Under specific temperature conditions, at 16 W, 18 W, 20 W, and 22 W, the number of fibroblasts increased to different degrees immediately after surgery (28.29±2.49, 30.97±3.57, 38.74±3.12, 45.68±4.27), 1 month after surgery (30.88±3.12, 32.46±4.02, 41.05±0.35, 50.41±0.08), and 3 months after surgery (29.99±2.01, 33.30±2.69, 39.00±3.29, 23.20±2.90) compared with that before surgery, and all were statistically significant (P < .05).

Conclusions: Our study clarifies that bipolar RF can decrease the skin and subcutaneous tissue thickness and increase the numbers of fibroblasts at the temperature of 36°C, 38°C, and 40°C and frequency of 16-22 W, which has a therapeutical effect on skin contraction. Our study might effectively improve the skin slack of patients, and the postoperative maintenance rate is high, and will not cause obvious complications. This study may provide a theoretical direction for clinicians to tighten the skin of patients using bipolar RF.

本刊更多论文

双极射频诱发皮肤收缩的实验研究。

背景:面部皮肤松弛已成为解决面部年轻化问题的重要组成部分。微创或无创紧肤手术已成为面部年轻化的趋势。双极射频(RF)是治疗皮肤松弛的新选择，比无手术切口的无创手术更有效。目的:探讨不同双极射频功率对家兔原箱面积、皮肤及皮下组织厚度变化及成纤维细胞数量的影响。设计:研究小组进行了动物实验。背景:本研究在南京中医药大学附属医院进行。实验对象:普通级成年新西兰兔18只(雌性，2.5-3.0公斤)。方法:在治疗区左侧对一只小兔子进行双极射频治疗。采用标准HE染色、Masson染色观察大鼠皮肤及皮下组织病理变化、原盒面积及成纤维细胞数量。观察指标:(1)观察术后即刻、术后1个月、术后3个月不同双极射频温度或不同双极射频功率下原盒面积、皮肤及皮下组织厚度变化、成纤维细胞数量。(2) HE、Masson染色标准结果。结果:在一定仪器功率的情况下，术后第36、38、40天，与术前比较，术后即刻(16.54±0.37、17.78±0.03、17.19±0.01)、术后1个月(16.59±0.31、17.82±0.01、18.34±0.30)、术后3个月(16.89±0.12、18.16±0.14、19.23±0.32)原盒面积均有不同程度缩小(P < 0.05)。在特定温度条件下，在16 W、18 W、20 W、22 W下，术后即刻(16.40±0.49、15.55±0.57、17.54±0.12、16.19±0.27)、术后1个月(16.88±0.12、17.46±0.02、18.05±0.35、19.41±0.08)、术后3个月(19.09±1.01、18.30±0.69、20.00±0.29、21.20±0.90)与术前比较，原盒面积均有不同程度的缩小(P < 0.05)。当功率固定时，不同温度下，术后即刻(6.7、6.8、7)、术后1个月(6、6.1、6.3)、术后3个月(6.4、6.5、6.2)皮肤和皮下组织厚度下降(P < 0.05)。温度固定时，不同功率下术后即刻(6.1、6.08、6.03)、术后1个月(6.2、6.15、6.13)、术后3个月(6.2、6.23、6.03)皮肤及皮下组织厚度均下降(P < 0.05)。在一定器械功率条件下，术后36 ~ 38d、40n时，术后即刻(26.54±2.37、30.78±3.03、37.19±4.01)、术后1个月(28.59±2.31、34.82±3.01、40.34±4.30)、术后3个月(30.89±0.12、38.16±0.14、42.23±0.32)与术前比较，成纤维细胞数量均有不同程度的增加，差异均有统计学意义(P < 0.05)。在特定温度条件下，16 W、18 W、20 W、22 W时，术后即刻(28.29±2.49、30.97±3.57、38.74±3.12、45.68±4.27)、术后1个月(30.88±3.12、32.46±4.02、41.05±0.35、50.41±0.08)、术后3个月(29.99±2.01、33.30±2.69、39.00±3.29、23.20±2.90)与术前比较，成纤维细胞数量均有不同程度的增加，差异均有统计学意义(P < 0.05)。结论:本研究表明，在36℃、38℃、40℃、16-22 W频率下，双极射频可降低皮肤和皮下组织厚度，增加成纤维细胞数量，对皮肤收缩具有治疗作用。本研究可有效改善患者皮肤松弛，术后维持率高，且不会出现明显并发症。本研究可为临床医生使用双极射频紧致患者皮肤提供理论指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Alternative therapies in health and medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

0.90

自引率

0.00%

发文量

219

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