Demonstration of phenolic compounds in plant tissues by an osmium-iodide postfixation procedure.

M Scalet, E Crivellato, F Mallardi
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引用次数: 38

Abstract

A simple procedure to stain phenols in plant tissues is described. Postfixation with an aqueous solution prepared by mixing 2 cc of 2% osmium tetroxide and 8 cc of 3% potassium iodide yields brilliant visualization of phenol-containing vacuoles in different tissues of plants (e.g., coffee, oak, tobacco and spruce) bearing high concentration of phenolic compounds. Areas bearing phenols become dark gray to black. Chemical experiments demonstrate that osmium-potassium iodide (Os-KI) mixture reacts rapidly with several naturally occurring plant phenols, developing black solutions from which black solids precipitate. Phenols containing omicron-dihydroxy groups react with Os-KI solution more rapidly than other structurally different phenols. Therefore, omicron-dihydroxy units in an aromatic ring seem to function as primary sites of reactivity with the osmium-iodide complexes.

用碘化锇后固定程序证明植物组织中的酚类化合物。
介绍了植物组织中酚类物质染色的一种简单方法。用2cc的2%四氧化锇和8cc的3%碘化钾混合而成的水溶液进行后固定,可以清晰地看到含有高浓度酚类化合物的植物(如咖啡、橡木、烟草和云杉)不同组织中的含酚液泡。含有酚的区域变成深灰色到黑色。化学实验表明,锇-碘化钾(Os-KI)混合物与几种天然存在的植物酚迅速反应,形成黑色溶液,从中沉淀黑色固体。含有双羟基基团的苯酚与Os-KI溶液的反应速度比其他结构不同的酚更快。因此,芳香环上的双羟基单位似乎是与碘化锇配合物反应的主要位点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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