Environmental DNA (eDNA) assays for the detection of Ridgway’s rail (Rallus obsoletus) in the United States

IF 0.9 4区环境科学与生态学 Q4 BIODIVERSITY CONSERVATION

Conservation Genetics Resources Pub Date : 2023-09-22 DOI:10.1007/s12686-023-01321-7

Xin Guan, Richard F. Lance, Sheena M. Feist

引用次数: 0

Abstract

Abstract We designed two novel environmental DNA (eDNA) assays for the detection of Ridgway’s Rail ( Rallus obsoletus ), and successfully validated each assay using eDNA samples collected from the species’ known distribution within the United States. These assays add to the suite of tools available for the monitoring of this rare and secretive marsh bird, and may help to further elucidate its movement patterns as well as identify important migration corridors. Observed sensitivity of the assays indicates exceptional performance, with limits of detection at ≤ 8 copies of the target eDNA fragment per reaction. Our publication adds to the growing body of literature supporting eDNA surveys as viable tools for bird monitoring endeavors.

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环境DNA (eDNA)检测Ridgway 's rail (Rallus obsoletus)在美国

摘要设计了两种检测Ridgway ' s Rail (Rallus obsoletus)的新型环境DNA (eDNA)检测方法，并利用该物种在美国已知分布的eDNA样本成功验证了每种检测方法的有效性。这些分析增加了一套可用的工具来监测这种稀有而神秘的沼泽鸟类，并可能有助于进一步阐明其运动模式以及确定重要的迁徙走廊。观察到的检测灵敏度表明了卓越的性能，每次反应的检测限≤8个目标eDNA片段。我们的出版物增加了越来越多的文献支持eDNA调查作为鸟类监测工作的可行工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Conservation Genetics Resources BIODIVERSITY CONSERVATION-GENETICS & HEREDITY

CiteScore

2.90

自引率

9.10%

发文量

审稿时长

3-6 weeks

期刊介绍： Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.