Carboxylesterase from edible legume seeds and their sensitivity to organophosphate and carbamate pesticides

IF 1.4 4区生物学 Q3 PLANT SCIENCES

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-08-31 DOI:10.15835/nbha51313234

Chiradet NASUK, Monrodee CHAOWARAT, Sumonwan CHUMCHUERE

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引用次数: 0

Abstract

Carboxylesterase from edible legume seeds, including black gram bean (Vigna mungo (L.) Hepper), mung bean (Vigna radiata (L.) Wilczek) and red bean (Vigna umbellata (Thunb.) Ohwi and Ohashi) has been partially purified using the ammonium sulphate salting-out procedure. The specific activity of the partially purified carboxylesterase extracted from black gram bean, mung bean and red bean are 3.96±0.26, 1.51±0.10 and 1.29±0.08 U/mg, respectively (p<0.05). The carboxylesterase from black gram bean showed very high sensitivity to 24 types of organophosphate pesticides (OPs) and 7 types of carbamate pesticides (CAs) at the concentrations of 0.001-3.0 µg/ml. The limit of detection (LOD) obtained for OPs and CAs pesticide residues were 0.002 and 0.01 mg/kg, respectively. For performance assessment of methods for pesticide determination in vegetables, all performance parameter values, including sensitivity, accuracy, and specificity of the carboxylesterase enzyme inhibition method, are 100% compared with commercial pesticide test kits and are 70.5%, 90%, and 100%, respectively, compared with chromatographic method analysis.

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食用豆科植物种子羧酸酯酶及其对有机磷和氨基甲酸酯类农药的敏感性

从食用豆类种子中提取羧酸酯酶，包括黑革兰豆(Vigna mungo (L.))绿豆(Vigna radiata (L.))(Wilczek)和红豆(Vigna umellata)。Ohwi和Ohashi)已经使用硫酸铵盐析程序部分纯化。从黑豆、绿豆和红豆中提取的部分纯化羧酸酯酶的比活性分别为3.96±0.26、1.51±0.10和1.29±0.08 U/mg (p < 0.05)。黑豆羧酸酯酶在0.001 ~ 3.0µg/ml范围内对24种有机磷农药(OPs)和7种氨基甲酸酯类农药(CAs)具有很高的敏感性。有机磷农药残留量的检出限(LOD)分别为0.002 mg/kg和0.01 mg/kg。对蔬菜中农药残留量测定方法进行性能评价，羧酸酯酶抑制法与市售农药检测试剂盒相比，灵敏度、准确度、特异度均为100%，与色谱法分析相比，灵敏度、准确度和特异度分别为70.5%、90%和100%。

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来源期刊

Notulae Botanicae Horti Agrobotanici Cluj-napoca PLANT SCIENCES-

CiteScore

2.70

自引率

0.00%

发文量

118

审稿时长

3 months

期刊介绍： Notulae Botanicae Horti Agrobotanici Cluj-Napoca is a peer-reviewed biannual journal aimed at disseminating significant research and original papers, critical reviews and short reviews. The subjects refer on plant biodiversity, genetics and plant breeding, development of new methodologies that can be of interest to a wide audience of plant scientists in all areas of plant biology, agriculture, horticulture and forestry. The journal encourages authors to frame their research questions and discuss their results in terms of the major questions of plant sciences, thereby maximizing the impact and value of their research, and thus in favor of spreading their studies outcome. The papers must be of potential interest to a significant number of scientists and, if specific to a local situation, must be relevant to a wide body of knowledge in life sciences. Articles should make a significant contribution to the advancement of knowledge or toward a better understanding of existing biological and agricultural concepts. An international Editorial Board advises the journal. The total content of the journal may be used for educational, non-profit purposes without regard to copyright. The distribution of the material is encouraged with the condition that the authors and the source (Notulae Botanicae Horti Agrobotanici Cluj-Napoca or JCR abbrev. title Not Bot Horti Agrobo) are mentioned.