Prevalence and molecular characterization of Cryptosporidium spp. in calves in the Siirt Province, Türkiye

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria Brno Pub Date : 2023-01-01 DOI:10.2754/avb202392030243

Özgür Yaşar Çelik, Tekin Şahin, Burçak Aslan Çelik, Özlem Orunç Kılınç, Adnan Ayan, Gürkan Akyıldız, Özge Oktay Ayan, Yaşar Göz, Kıvanç İrak, Gül Görmez

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Abstract

Cryptosporidiosis, one of the main protozoan infections of the last century, is especially dangerous for calves and causes significant economic losses. This research was carried out to determine the prevalence of Cryptosporidium spp. by microscopic and molecular methods and to determine subtypes in 100 calves up to 6 months old and with diarrhoea in the Siirt Province, Türkiye. As a result of the microscopic examination (Kinyoun’s acid-fast), Cryptosporidium spp. oocysts were found in 8 (8%) of 100 samples. As a result of nested PCR, 826-864 bp specific bands for Cryptosporidium spp. were obtained in 13 (13%) of 100 samples. When the DNA sequences of the SSU rRNA gene were compared with the NCBI Basic Local Alignment Search Tool database, it was determined that eight samples sequence analyses showed 100% similarity with the C. parvum , C. ryanae , and C. bovis samples. The detection of C. parvum , which has zoonotic importance in this study, suggests that calves with diarrhoea may be a source of contamination for other animals and humans. Therefore, animal owners and people in close contact with animals should be informed about the public health of cryptosporidiosis.

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新疆锡伊特省犊牛隐孢子虫流行病学及分子特征分析

隐孢子虫病是上个世纪主要的原生动物感染之一，对小牛尤其危险，并造成重大经济损失。开展这项研究是为了通过显微镜和分子方法确定隐孢子虫的流行情况，并确定基耶省锡尔特省100头6个月以下腹泻小牛的亚型。镜检(金扬氏抗酸法)结果显示，100份样本中有8份(8%)发现隐孢子虫卵囊。巢式PCR结果显示，100份样品中有13份(13%)获得了826 ~ 864 bp的隐孢子虫特异条带。将SSU rRNA基因的DNA序列与NCBI Basic Local Alignment Search Tool数据库进行比对，确定8个样本序列分析与C. parvum、C. ryanae和C. bovis样本的相似性为100%。在本研究中具有人畜共患重要性的细小梭菌的检测表明，患有腹泻的小牛可能是其他动物和人类的污染源。因此，应向动物主人和与动物密切接触的人通报隐孢子虫病的公共卫生情况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Veterinaria Brno 农林科学-兽医学

CiteScore

1.00

自引率

33.30%

发文量

审稿时长

18-36 weeks

期刊介绍： ACTA VETERINARIA BRNO is a scientific journal of the University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic. The scientific journal Acta Veterinaria Brno is dedicated to the publication of original research findings and clinical observations in veterinary and biomedical sciences. Original scientific research articles reporting new and substantial contribution to veterinary science and original methods that have not been submitted for publication elsewhere are considered for publication. A written statement to this effect should accompany the manuscript, along with approval for publication by the author´s head of department. The authors bear full responsibility for the contents of their contribution. Book reviews are published, too.