Copper-Zinc Superoxide Dismutase (CuZn-SOD) Electrochemical Catalytic Amplification Sensing at Pt Ultramicroelectrodes

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-10-31 DOI:10.29356/jmcs.v67i4.1963

Annelis O. Sánchez-Álvarez, J. Andres Melendez, Mariena Silvestry-Ramos, Carlos R. Cabrera

{"title":"Copper-Zinc Superoxide Dismutase (CuZn-SOD) Electrochemical Catalytic Amplification Sensing at Pt Ultramicroelectrodes","authors":"Annelis O. Sánchez-Álvarez, J. Andres Melendez, Mariena Silvestry-Ramos, Carlos R. Cabrera","doi":"10.29356/jmcs.v67i4.1963","DOIUrl":null,"url":null,"abstract":"Abstract. Copper/zinc superoxide dismutase (CuZnSOD), a 32.5 kDa metalloprotein with a radius of ca. 2.1 nm, catalyses the superoxide to hydrogen peroxide and molecular oxygen. At the femtomolar concentration range, has been sensed through electrochemical catalytic amplification using a Pt ultramicroelectrode. During amperometric (i vs. t) analysis, cathodic and anodic current transitions peaks were seen, in agreement with the metalloprotein catalytic mechanism. The current amplitudes were analyzed and correspond to the CuZnSOD dimensions. Thermal treatment of metalloprotein samples at 80 °C showed larger current spikes suggesting aggregation without losing its catalytic capability. The size was confirmed by transmission electron microscopy. Resumen. Cuprozinc superóxido dismutasa (CuZnSOD), es una metaloproteina de 32.5 kDa con un radio de aproximadamente 2.1 nm. Esta enzima cataliza la reacción de superóxido a peróxido y oxígeno molecular. Por primera vez, esta proteína es detectada a concentraciones femtomolares haciendo uso de la técnica electroquímica conocida como amplificación catalítica y la tecnología de ultra-microelectrodos de Pt. Durante un análisis amperométrico (curvas i vs. t), se observaron picos transitorios de corriente catódica y anódica que concuerdan con el mecanismo catalítico de la enzima. Al analizar la amplitud de la corriente, la misma concuerda con las dimensiones de CuZnSOD. Luego de exponer la proteína a un tratamiento térmico de 80 °C, CuZnSOD mostró picos de corriente transitorias que sugieren aglomeración de la enzima sin perder su capacidad catalítica. El tamaño fue confirmado por microscopía electrónica de transmisión.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29356/jmcs.v67i4.1963","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract. Copper/zinc superoxide dismutase (CuZnSOD), a 32.5 kDa metalloprotein with a radius of ca. 2.1 nm, catalyses the superoxide to hydrogen peroxide and molecular oxygen. At the femtomolar concentration range, has been sensed through electrochemical catalytic amplification using a Pt ultramicroelectrode. During amperometric (i vs. t) analysis, cathodic and anodic current transitions peaks were seen, in agreement with the metalloprotein catalytic mechanism. The current amplitudes were analyzed and correspond to the CuZnSOD dimensions. Thermal treatment of metalloprotein samples at 80 °C showed larger current spikes suggesting aggregation without losing its catalytic capability. The size was confirmed by transmission electron microscopy. Resumen. Cuprozinc superóxido dismutasa (CuZnSOD), es una metaloproteina de 32.5 kDa con un radio de aproximadamente 2.1 nm. Esta enzima cataliza la reacción de superóxido a peróxido y oxígeno molecular. Por primera vez, esta proteína es detectada a concentraciones femtomolares haciendo uso de la técnica electroquímica conocida como amplificación catalítica y la tecnología de ultra-microelectrodos de Pt. Durante un análisis amperométrico (curvas i vs. t), se observaron picos transitorios de corriente catódica y anódica que concuerdan con el mecanismo catalítico de la enzima. Al analizar la amplitud de la corriente, la misma concuerda con las dimensiones de CuZnSOD. Luego de exponer la proteína a un tratamiento térmico de 80 °C, CuZnSOD mostró picos de corriente transitorias que sugieren aglomeración de la enzima sin perder su capacidad catalítica. El tamaño fue confirmado por microscopía electrónica de transmisión.

查看原文本刊更多论文

铜锌超氧化物歧化酶(CuZn-SOD)在Pt超微电极上的电化学催化放大传感

摘要铜锌超氧化物歧化酶(CuZnSOD)是一种分子量为32.5 kDa、半径约2.1 nm的金属蛋白，可催化超氧化物生成过氧化氢和分子氧。在飞摩尔浓度范围内，通过使用铂超微电极的电化学催化放大进行了感应。在安培(i vs. t)分析中，观察到阴极和阳极电流转变峰，与金属蛋白的催化机制一致。分析了电流幅值，并与CuZnSOD尺寸相对应。金属蛋白样品在80°C下热处理时显示出较大的电流峰，表明其聚集而不失去催化能力。透射电镜证实了其大小。Resumen。铜prozinc superóxido dismutasa (CuZnSOD)是一种金属蛋白，在约2.1 nm的射电光谱下，de为32.5 kDa。催化enzima cataliza reacción de superóxido a peróxido y oxígeno分子。1 .首先，请注意proteína微型微型探测器和微型微型微型探测器和微型微型微型微型探测器和微型微型微型微型探测器和微型微型微型探测器和微型微型微型探测器和微型微型微型探测器和微型微型探测器和微型微型探测器和微型微型探测器。相似的振幅，一致的振幅，一致的振幅，一致的量纲，一致的量纲，一致的量纲。Luego de exponer la proteína a untratimento de 80°C, CuZnSOD mostró picos de corriente transioriente que sugieren aglomeración de la enzima sin perder sucapacidad catalítica。El tamaño fue confirmado por microscopía electrónica de transmisión。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.