Evaluation of the polymorphism of the GDF9 and BMP15 genes and their relationship with the reproductive functions of sheep of different breeds

IF 0.3 Q4 ECOLOGY
Z. K. Gadzhiev, E. S. Surzhikova, D. D. Evlagina, A. I. Surov, S. N. Shumaenko
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Abstract

Aim . Polymorphism is the result of evolutionary processes and is inherited, associated with biodiversity and modified by natural selection and functions to preserve various forms of population. It is economically advantageous to find genes that can be used in breeding in such a way as to increase the fertility of animals in different natural geographical zones, since the adaptive abilities of organisms are complicated. In this regard, the purpose of this study was to study the genetic potential of sheep of different breeds bred in various ecological and geographical regions of the North Caucasus with pasture-pasture and pasture-stall conditions of sheep breeding. Material and Methods . Genotyping was undertaken of sheep of different breeds contained in various natural and climatic zones of the Republic of Dagestan and the most arid region of Stavropol Territory in the zone of risky agriculture where the climate is sharply continental. Genotyping in the loci of the genes of differential growth factor (GDF9) and bone morphogenetic protein (BMP15) was carried out by PCR-PDRF (polymorphism of the lengths of restriction fragments) by cutting DNA using endonuclease restriction and further analysis of the size of the resulting PCR fragments. The genetic structure of genes has been studied by methods of genetic and statistical analysis of the data obtained. Conclusion . The genetic structure of sheep populations of different breeds bred in the foothills of the Republic of Dagestan and the arid region of Stavropol Territory was studied for the first time. Information obtained on the role of the degree of genetic variability of sheep of different breeds provides data which can contribute to the ecological well-being of herds and the status characterising the signs of multiple birth rates is also determined. This will further improve and significantly accelerate breeding work with livestock, since an important task of further increasing the efficiency of the industry is the reproduction of the herd together with a simultaneous increase in the productivity of animals.
不同品种绵羊GDF9和BMP15基因多态性及其与生殖功能的关系
的目标。多态性是进化过程的结果,是遗传的,与生物多样性有关,并通过自然选择进行修改,并具有保存各种形式的种群的功能。由于生物的适应能力是复杂的,因此寻找可用于育种的基因以增加不同自然地理区域动物的繁殖力在经济上是有利的。因此,本研究的目的是研究北高加索不同生态地理区域在放牧-放牧和放牧-圈养条件下饲养的不同品种绵羊的遗传潜力。材料和方法。对达吉斯坦共和国不同自然气候带和斯塔夫罗波尔领土最干旱地区的不同品种的绵羊进行了基因分型,该地区属于气候明显大陆性的危险农业区。采用PCR- pdrf(限制性片段长度多态性)技术,对不同生长因子(GDF9)和骨形态发生蛋白(BMP15)基因位点进行基因分型,并进一步分析所得PCR片段的大小。通过对所得资料的遗传和统计分析方法,对基因的遗传结构进行了研究。结论。首次对达吉斯坦共和国山麓和斯塔夫罗波尔地区干旱地区不同品种绵羊种群的遗传结构进行了研究。所获得的关于不同品种绵羊遗传变异程度的作用的信息提供了有助于畜群生态福祉的数据,并确定了具有多胎出生率特征的状况。这将进一步改善并显著加快牲畜育种工作,因为进一步提高该行业效率的一项重要任务是在提高动物生产力的同时实现畜群的繁殖。
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来源期刊
CiteScore
0.80
自引率
50.00%
发文量
73
审稿时长
8 weeks
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