Lateral bud induction and establishment of regeneration system of cassia mimosoides L.

IF 0.4 4区生物学 Q4 PLANT SCIENCES

Bangladesh Journal of Botany Pub Date : 2023-08-31 DOI:10.3329/bjb.v52i20.68214

None Ya Li Yang, None Qian Qian Dai, None Pei Ting He, Zhan Wen Lin, Sheng Yuan Zhang, Da Du Lin, None Ying Liu, Ming Zhai, Lu Bin Zhang

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引用次数: 0

Abstract

To establish an efficient plant regeneration system of Cassia mimosoides, the 10-day-old seedlings were used as explants for inducing lateral buds. Results showed that the addition of BA and NAA for different ratios on the basis of 1/2 MS could effectively induce the production of lateral buds. By adding different additional elements, it was found that the growth of lateral buds was longer and stronger on the medium of 1/2 MS + 3.0 mg/l BA + 0.1 mg/l NAA + 4.0 mg/l Gln or 1/2 MS + 3.0 mg/l BA + 0.1 mg/l NAA + 1.0 mg/l AgNO3. Meanwhile, cutting with the lateral buds of tissue culture seedling, the rooting rate could reach 77.8% when 1/2 MS medium contained 0.2 mg/l NAA, although the root length was shorter. When the regenerated strong seedlings were transplanted into the soil, the survival rate was over 75%. Therefore, the best medium for lateral bud induction using 10-day-old seedlings as explants in C. mimosoides was 1/2 MS + 3.0 mg/l BA + 0.1 mg/l NAA. The growth of lateral buds can be promoted by the appropriate concentration of Gln and AgNO3, and the best rooting medium was 1/2 MS contained 0.2 mg/l NAA. Bangladesh J. Bot. 52(2): 515-521, 2023 (June) Special

查看原文本刊更多论文

决明子侧芽诱导及再生体系的建立。

为建立高效的含水决明子植株再生体系，以10 d龄的决明子幼苗为外植体诱导侧芽。结果表明，在1/2 MS的基础上，添加不同比例的BA和NAA均能有效诱导侧芽的产生。通过添加不同元素，发现在1/2 MS + 3.0 mg/l BA + 0.1 mg/l NAA + 4.0 mg/l Gln或1/2 MS + 3.0 mg/l BA + 0.1 mg/l NAA + 1.0 mg/l AgNO3培养基上侧芽生长得更长、更强。以组培苗侧芽扦插，在1/2 MS培养基中NAA含量为0.2 mg/l时，根长较短，但生根率可达77.8%。将再生的壮苗移栽到土壤中，成活率可达75%以上。因此，以10日龄种子为外植体诱导侧芽的最佳培养基为1/2 MS + 3.0 mg/l BA + 0.1 mg/l NAA。适宜浓度的Gln和AgNO3均能促进侧芽的生长，生根培养基以含0.2 mg/l NAA的1/2 MS为最佳。[j] .植物学报，52(2):515-521,2023(6):特刊

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来源期刊

Bangladesh Journal of Botany 生物-植物科学

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

9 months

期刊介绍： Bangladesh in situated on the north of Bay of Bengal. Climatically it is a humid subtropical country. Most of the land is deltaic plain of two great rivers, the Ganges and the Bhrammaputra and it tributaries. The country has rich diversity of plants. Main crops cultivated are Rice, Jute, Wheat, Maize, Sugarcane, Mustard and different kinds of Lentils. There are a good number of Public and Private Universities and Plant Research Establishments. Bangladesh Journal of Botany is the official organ of the Bangladesh Botanical Society established in 1972. Since 1972 Bangladesh Journal of Botany is being published regularly. Two issues of the Journal are published, one in June and another in December. Scientific papers (Full paper and short communication) on any field of Plant Sciences from anywhere in the World are considered for publication in Bangladesh Journal of Botany.