Xenorhabdus bakwenae sp. n., associated with the entomopathogenic nematode Steinernema bakwenae

IF 1.2 4区生物学 Q2 ZOOLOGY

Nematology Pub Date : 2023-10-18 DOI:10.1163/15685411-bja10284

Carla L. Ritter, Antoinette P. Malan, Leon M.T. Dicks

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引用次数: 0

Abstract

Summary The bacterial strain SF857 was isolated from the entomopathogenic nematode, Steinernema bakwenae SF857, found in soil samples of St Lucie cherry, a species of cherry tree, from the Muriti farm, close to Kroondal (25°44′E, 59°99′S) in the northwest province of South Africa. Strain SF857 is Gram-negative, catalase and oxidase-negative, and lecithinase-positive. Strain SF857 shared the highest 16S rRNA gene sequence similarity (97.7%) with the type strain of Xenorhabdus ishibashii . However, a comparison of housekeeping gene sequences showed that strain SF857 grouped closer to X. griffiniae , X. ehlersii and X. thuongxuanensis than to X. ishibashii . Digital DNA-DNA hybridisation (dDDH) between strain SF857 and all Xenorhabdus spp. is less than the 70% species threshold, confirming that the strain belongs to a separate species. Furthermore, the biochemical characteristics of strain SF857 differ from other Xenorhabdus spp. Based on these findings, strain SF857 represents a novel species within the genus Xenorhabdus , for which the name Xenorhabdus bakwenae sp. n. (type strain SF857 T ) is proposed. Pathogenicity bioassays with last-instar Cydia pomonella showed S. bakwenae to be highly virulent, with potential use as a biocontrol agent in apple orchards.

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bakwenae Xenorhabdus sp. n.，与昆虫病原线虫bakwenae steinnema有关

菌株SF857是从南非西北省靠近Kroondal(25°44′e, 59°99′s)的Muriti农场的圣露西樱桃(St Lucie cherry)土壤样品中分离到的昆虫病原线虫bakwenae steinerma SF857分离得到的。菌株SF857革兰氏阴性，过氧化氢酶和氧化酶阴性，卵磷脂酶阳性。菌株SF857与石桥Xenorhabdus ishibashii型菌株16S rRNA基因序列相似性最高(97.7%)。然而，整理基因序列的比较表明，菌株SF857更接近于X. griffiniae, X. ehlersii和X. thongxuanensis，而不是X. ishibashii。菌株SF857与所有Xenorhabdus属之间的数字DNA-DNA杂交(dDDH)小于70%的种阈值，证实该菌株属于单独的种。此外，菌株SF857的生化特征与其他Xenorhabdus属不同，因此，SF857是Xenorhabdus属的一个新种，并被命名为Xenorhabdus bakwenae sp. n.(类型菌株SF857 T)。用末龄波蒙Cydia pomonella进行的致病性生物测定表明，bakwenae具有很强的毒力，具有作为苹果园生物防治剂的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nematology 生物-动物学

CiteScore

2.60

自引率

33.30%

发文量

审稿时长

3 months

期刊介绍： Nematology is an international journal for the publication of all aspects of nematological research (with the exception of vertebrate parasitology), from molecular biology to field studies. Papers on nematode parasites of arthropods, and on soil free-living nematodes, and on interactions of these and other organisms, are particularly welcome. Research on fresh water and marine nematodes is also considered when the observations are of more general interest. Nematology publishes full research papers, short communications, Forum articles (which permit an author to express a view on current or fundamental subjects), perspectives on nematology, and reviews of books and other media.