Iraqi Hyacinthus orientalis L. Flowers as the Source of Bioactive Compounds Especially Stigmasterol: Identification, Isolation and Characterization

Abstract

This research concentrated on the isolation and identification of stigmasterol from cultivated Iraqi Hyacinthus orientalis flowers part, as there are few studies that deal with these plant-specific flowers as e-research shows. Extraction were done for the flowers part of plant, by the soxhlet apparatus using 85% ethanol, followed by fractionation with petroleum ether to obtain the target of this study. Phytochemical screening was done by gas chromatography-mass spectrometry (GC-MS) for petroleum ether fraction qualitative and quantitative estimation and isolation of stigmasterol by reverse phase high-performance liquid chromatography (RP-HPLC), identification of isolated stigmasterol by Fourier transforms infrared (FTIR) spectroscopy, ultraviolet spectroscopy and liquid chromatography-mass spectroscopy (LC-MS). Announcing that the study considered the first to isolate stigmasterol from flowers part of Iraqi H. orientalis.