Complete viral protein coding genes NS1 and VP1 sequences and phylogenetic analysis of a novel recombinant Mink Enteritis Virus isolated in China

IF 0.2 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Indian Journal of Animal Sciences Pub Date : 2023-10-31 DOI:10.56093/ijans.v93i11.133463

TIANFEI YU, CHEN SUN, MING LI

引用次数: 0

Abstract

The primary objective of this study was to detect the presence of MEV in mink samples and investigate the genetic characteristics of the virus. In 2017, a newly identified strain of MEV, named MEV-HLJ, was isolated. The virus’s complete NS1 and VP1 gene sequences were amplified using PCR and subjected to phylogenetic analysis. The results indicated that despite moderate virulence, MEV- HLJ belonged to the attenuated group. Recombination analysis using the GARD programme identified a putative recombination breakpoint at nucleotide position 2,075 between the NS1 and VP1 genes. Separate phylogenetic trees were constructed to confirm the recombinant event. This study provides insights into the genetic diversity and evolution of MEV isolates.

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一株重组水貂肠炎病毒蛋白编码基因NS1和VP1序列及系统发育分析

本研究的主要目的是检测水貂样本中MEV的存在，并研究该病毒的遗传特征。2017年，一种新发现的MEV菌株被分离出来，命名为MEV- hlj。采用PCR扩增病毒NS1和VP1完整基因序列，并进行系统发育分析。结果表明，MEV- HLJ虽然毒力适中，但属于弱毒组。使用GARD程序进行重组分析，在NS1和VP1基因之间的核苷酸位置2075处确定了推定的重组断点。构建单独的系统发育树来证实重组事件。这项研究为MEV分离株的遗传多样性和进化提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Animal Sciences 农林科学-奶制品与动物科学

CiteScore

0.60

自引率

25.00%

发文量

220

审稿时长

8 months

期刊介绍： Articles published in The Indian Journal of Animal Sciences encompass a broad range of research topics in animal health and production related to cattle, buffalo, sheep, goat, camel, equines, pig, rabbit, yak, mithun, poultry and fisheries. Studies involving wildlife species and laboratory animal species that address fundamental questions about their biology will also be considered for publication. All manuscripts must present some new development and must be original, timely, significant and scientifically excellent. Papers will be rejected if standards of care of, or procedures performed on animals are not up to those expected of humane veterinary scientists. At a minimum, standards must meet the International Guiding Principles for Biomedical Research involving Animals, as issued by the Council for International Organizations of Medical Sciences. (C.I.O.M.S., c/o WHO, CH 1211 Geneva 27, Switzerland). Articles reporting new animal disease must follow GOI directive as given in detail in Guidelines to Authors.