Falah Saleh Mohammed, Imran Uysal, Ali Erdem Sabik, Muhittin Dogan, Mustafa Sevindik
{"title":"TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIMICROBIAL AND ANTIOXIDANT POTENTIALS OF CAMPANULA STRIGOSA BANKS & SOL","authors":"Falah Saleh Mohammed, Imran Uysal, Ali Erdem Sabik, Muhittin Dogan, Mustafa Sevindik","doi":"10.55251/jmbfs.10057","DOIUrl":null,"url":null,"abstract":"Humans find several uses for plants. Not only is it good for you from a nutritional standpoint, but it has also been used to cure a wide range of illnesses. In this research, we analysed Campanula strigosa Banks & Sol. for its antioxidant and antibacterial properties. Total phenolic and flavonoid concentrations were also calculated. For this purpose, a soxhlet apparatus was used to extract ethanol and methanol from the plant's above-ground portions. The effectiveness of antioxidants was measured using Rel Assay kits. The agar dilution technique was used to test for antimicrobial activity against various bacterial and fungus species. Folin-Ciocalteu reagent was used to quantify total phenolic content. An aluminium chloride assay determined the total flavonoid concentration. Studies found that the plant extract had a TAS value of 4.974±0.259 mmol/L, a TOS value of 12.437±0.150 µmol/L, and an OSI value of 0.251±0.014. The plant extract was shown to be effective against bacterial strains at concentrations between 100 and 400 µg/mL, and against fungal strains at concentrations between 50 and 100 µg/mL. Ethanol extract also had a greater total phenolic concentration and total flavonoid content. C. strigosa was shown to have antibacterial and antioxidant properties in this study.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Microbiology, Biotechnology and Food Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.55251/jmbfs.10057","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Humans find several uses for plants. Not only is it good for you from a nutritional standpoint, but it has also been used to cure a wide range of illnesses. In this research, we analysed Campanula strigosa Banks & Sol. for its antioxidant and antibacterial properties. Total phenolic and flavonoid concentrations were also calculated. For this purpose, a soxhlet apparatus was used to extract ethanol and methanol from the plant's above-ground portions. The effectiveness of antioxidants was measured using Rel Assay kits. The agar dilution technique was used to test for antimicrobial activity against various bacterial and fungus species. Folin-Ciocalteu reagent was used to quantify total phenolic content. An aluminium chloride assay determined the total flavonoid concentration. Studies found that the plant extract had a TAS value of 4.974±0.259 mmol/L, a TOS value of 12.437±0.150 µmol/L, and an OSI value of 0.251±0.014. The plant extract was shown to be effective against bacterial strains at concentrations between 100 and 400 µg/mL, and against fungal strains at concentrations between 50 and 100 µg/mL. Ethanol extract also had a greater total phenolic concentration and total flavonoid content. C. strigosa was shown to have antibacterial and antioxidant properties in this study.