Chemical Profiling on Bioactive Stilbenoids in the Seeds of Paeonia Species Growing Wild in Greece

IF 2.5 4区工程技术 Q3 CHEMISTRY, ANALYTICAL

Separations Pub Date : 2023-10-13 DOI:10.3390/separations10100540

Eleni Dimitropoulou, Konstantia Graikou, Vithleem Klontza, Ioanna Chinou

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引用次数: 0

Abstract

The seeds of Paeonia clusii Stern subsp. clusii and Paeonia mascula (L.) Mill. subsp. mascula growing wild in Greece, though not previously investigated, has been studied as a source of bioactive stilbenoids and other phenolics. Their methanol extracts were analyzed using ultra high-performance liquid chromatography—high-resolution mass spectrometry (UHPLC-HRMS), and among the identified metabolites (62), 19 paeoniflorin’s derivatives, 17 flavonoids and 12 stilbenes were detected. Moreover, through classic phytochemical separation procedures, twelve among them were isolated and fully spectrally determined as trans-resveratrol, trans-resveratrol-4′-O-β-D-glucopyranoside, cis-resveratrol-4′-O-β-D-glucopyranoside, trans-gnetin-H, trans-ε-viniferin, luteolin, luteolin-3′-O-β-D-glucopyranoside, luteolin-3′,4′-di-O-β-D-glucopyranoside, apigenin, hispidulin, paeoniflorin and benzoyl-paeoniflorin. All seed extracts were measured for their total phenolic content (TPC), appearing as a rich source (116.04 and 103.63 mg GAE/g extract, respectively), followed by free radical (DPPH) scavenging capacity (75.24% and 91.54% inhibition at the concentration of 200 μg/mL). The evaluation of tyrosinase inhibition for both extracts (61% and 70%, respectively) confirmed the potential for their future application in skin health care, comparable with other paeonies of Chinese origin, which are well-known as skin whitening and anti-aging promoters.

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希腊野生芍药种子中生物活性Stilbenoids的化学分析

芍药的种子。赤芍和芍药(L.)轧机。无性系种群。在希腊野生生长的阳刚草，虽然以前没有被调查过，但已经被研究作为生物活性苯乙烯类化合物和其他酚类物质的来源。采用超高效液相色谱-高分辨率质谱(UHPLC-HRMS)对其甲醇提取物进行分析，鉴定出62种代谢物，其中芍药苷衍生物19种，类黄酮17种，二苯乙烯12种。通过经典的植物化学分离方法，分离得到12种化合物，分别为反式白藜芦醇、反式白藜芦醇-4′-O-β- d -葡萄糖苷、顺式白藜芦醇-4′-O-β- d -葡萄糖苷、反式皂素- h、反式ε-葡萄糖苷、木犀草素、木犀草素-3′-O-β- d -葡萄糖苷、木犀草素-3′、4′- 2 -O-β- d -葡萄糖苷、芹菜素、hispidulin、芍药苷和苯甲酰芍药苷。所有种子提取物的总酚含量(TPC)均为丰富的来源(分别为116.04和103.63 mg GAE/g提取物)，其次是自由基(DPPH)清除能力(200 μg/mL浓度下抑制75.24%和91.54%)。两种提取物的酪氨酸酶抑制率(分别为61%和70%)证实了它们在皮肤保健方面的潜在应用前景，与其他中国原产的促进皮肤美白和抗衰老的芍药相当。

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来源期刊

Separations Chemistry-Analytical Chemistry

CiteScore

3.00

自引率

15.40%

发文量

342

审稿时长

12 weeks

期刊介绍： Separations (formerly Chromatography, ISSN 2227-9075, CODEN: CHROBV) provides an advanced forum for separation and purification science and technology in all areas of chemical, biological and physical science. It publishes reviews, regular research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, unique features of this journal: Manuscripts regarding research proposals and research ideas will be particularly welcomed. Electronic files and software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material. Manuscripts concerning summaries and surveys on research cooperation and projects (that are funded by national governments) to give information for a broad field of users. The scope of the journal includes but is not limited to: Theory and methodology (theory of separation methods, sample preparation, instrumental and column developments, new separation methodologies, etc.) Equipment and techniques, novel hyphenated analytical solutions (significantly extended by their combination with spectroscopic methods and in particular, mass spectrometry) Novel analysis approaches and applications to solve analytical challenges which utilize chromatographic separations as a key step in the overall solution Computational modelling of separations for the purpose of fundamental understanding and/or chromatographic optimization