Are Buffy-coat Pooled Platelet Concentrates an Effective Alternative to Apheresis Platelet Concentrates? An In vitro Analysis at a Tertiary Care Center in Northern India

IF 0.8 Q3 MEDICINE, GENERAL & INTERNAL
Prashant Agarwal, Ashish Jain, Priti Elhence, Anupam Verma
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Abstract

Background: There is a need for platelet products to have the best quality. Apheresis platelet concentrates (PCs) obtained from single-donors PCs (SD-PCs) are considered best but have issues such as feasibility and cost. Buffy-coat pooled PCs (BCP-PCs) are considered an alternative to SD-PCs. This study compares BCP-PCs and SD-PCs for in vitro quality parameters and their changes during storage. Materials and Methods: Fifteen units of BCP-PCs and 15 units of SD-PCs were prepared. In this study, a pool of five buffy coats was prepared. Fifteen units of BCP-PCs were analyzed on day 1 and day 5 of storage, while 15 SD-PCs were analyzed on day 1 while ten units on day 5. The parameters analyzed were volume, hematological parameters, pH, swirling, and sterility. Results: The mean platelets count of SD-PCs was found to be significantly higher as compared to BCP-PCs. White blood cells (WBCs) contamination was significantly lower in BCP-PCs as compared to SD-PCs. The mean pH and mean platelet volume of SD-PCs were significantly lower than BCP-PCs. During storage, the mean platelets count of BCP-PCs was decreased significantly while that of SD-PCs nonsignificantly. The mean WBCs count and pH decreased in both BCP-PCs and SD-PCs significantly. All units in both types of PCs were sterile. Conclusion: Platelet yield was significantly better in SD-PCs, while mean WBCs contamination was significantly lower in BCP-PCs. BCP-PCs may be preferred in place of SD-PCs in case of nonavailability of apheresis, difficulty in finding a willing donor, or when the cost is of consideration.
褐皮池血小板浓缩物是一种有效的血小板浓缩物替代单采血小板浓缩物吗?在印度北部三级保健中心的体外分析
背景:血小板产品需要有最好的质量。单供血小板浓缩液(SD-PCs)被认为是最好的,但存在可行性和成本等问题。Buffy-coat pool pc (bcp - pc)被认为是sd - pc的替代品。本研究比较了BCP-PCs和SD-PCs的体外质量参数及其在贮存期间的变化。材料与方法:制备BCP-PCs 15个单位,SD-PCs 15个单位。在这项研究中,准备了一个由5只灰褐色外套组成的水池。BCP-PCs在第1天和第5天分析15个单位,SD-PCs在第1天分析15个单位,在第5天分析10个单位。分析的参数包括体积、血液学参数、pH、旋流和无菌性。结果:SD-PCs的平均血小板计数明显高于BCP-PCs。与SD-PCs相比,BCP-PCs的白细胞污染显著降低。SD-PCs的平均pH值和平均血小板体积显著低于BCP-PCs。储存期间,BCP-PCs的平均血小板计数显著下降,而SD-PCs的平均血小板计数无显著下降。BCP-PCs和SD-PCs的平均白细胞计数和pH均显著降低。两种pc机的所有单元都是无菌的。结论:SD-PCs的血小板产率明显较高,而BCP-PCs的白细胞平均污染明显较低。在无采血、难以找到自愿供体或考虑到成本的情况下,BCP-PCs可能优于SD-PCs。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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