Novel Approach to Investigate the Effect of High-Dose Methylprednisolone on Erythrocyte Morphology: White Light Diffraction Microscopy

IF 1 4区工程技术 Q4 INSTRUMENTS & INSTRUMENTATION

Measurement Science Review Pub Date : 2023-10-01 DOI:10.2478/msr-2023-0026

Cigdem Deniz, Ozlem Kocahan, Bengu Altunan, Aysun Unal

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Abstract

Abstract The present study focuses on quantitative phase imaging of erythrocytes with the aim to evaluate the effects of high-dose methylprednisolone (HDMP) on erythrocytes in vivo under physiological conditions in human blood samples. Samples from ten patients, prescribed to be treated with 1000 mg/day intravenous methylprednisolone for 5 days, were analyzed by white light diffraction phase microscopy (WDPM) for quantitative imaging. WDPM, an optical measurement technique, enables single shot measurement and low speckle noise using white light. Quantitative phase imaging performed with this experimental setup allowed the determination of erythrocyte morphology with 9 different parameters. In vivo quantitative analysis of erythrocytes by WDPM, which is a simple and reliable method, shows that HDMP treatment has no significant effect on erythrocyte morphology. With the developing technology, interdisciplinary studies on individuals under treatment should play an important role in elucidating the interaction between steroids and erythrocytes.

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研究大剂量甲基强的松龙对红细胞形态影响的新方法:白光衍射显微镜

摘要本研究主要通过红细胞的定量相成像技术，探讨高剂量甲基强的松龙(HDMP)在生理条件下对人血样本体内红细胞的影响。10例患者给予1000mg /d甲基强的松龙静脉注射5天，采用白光衍射相显微镜(WDPM)进行定量成像。WDPM是一种光学测量技术，可以使用白光进行单次测量和低散斑噪声。定量相成像进行了这个实验设置允许红细胞形态的测定与9个不同的参数。用WDPM对红细胞进行体内定量分析，是一种简单可靠的方法，结果表明HDMP处理对红细胞形态无明显影响。随着技术的发展，对治疗个体的跨学科研究将在阐明类固醇与红细胞之间的相互作用方面发挥重要作用。

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来源期刊

Measurement Science Review INSTRUMENTS & INSTRUMENTATION-

CiteScore

2.00

自引率

11.10%

发文量

审稿时长

4.8 months

期刊介绍： - theory of measurement - mathematical processing of measured data - measurement uncertainty minimisation - statistical methods in data evaluation and modelling - measurement as an interdisciplinary activity - measurement science in education - medical imaging methods, image processing - biosignal measurement, processing and analysis - model based biomeasurements - neural networks in biomeasurement - telemeasurement in biomedicine - measurement in nanomedicine - measurement of basic physical quantities - magnetic and electric fields measurements - measurement of geometrical and mechanical quantities - optical measuring methods - electromagnetic compatibility - measurement in material science