Other Sea Star Igkappa Gene Cloning Assay in E.Coli with New Parameters

Abstract

The plasmid vector pET-28b(+) named “Young” was produced according a work of 2014 [1]. This construct is designed to allow the expression of a 13.6 kDA protein with a C-terminal 6histag. It is supposed to be an anti-HRP (Horse-radish peroxydase protein). This protein was not expressed in first E.coli: we attempt to explain this phenomenon.