Expression levels and diagnostic value of serum GDNF, CEA and CA199 in patients with Colorectal Carcinoma

IF 2 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jue Wang, Lulu Liu, Yan Zheng, Sai Gu
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Abstract

Background: To investigate the expression levels and diagnostic value of glial cell line-derived neurotrophic factor (GDNF), carcinoembryonic antigen (CEA) and carbohydrate antigen199 (CA199) in patients with colorectal carcinoma (CRC). Methods. 50 CRC patients at our hospital from Feb. 2020 to Feb. 2021 were chosen as the malignant group, another 50 patients with benign colonic diseases were chosen as the benign group, and 50 healthy people who came to our hospital for physical examination during the same period were considered as the control group. Fasting peripheral venous blood was taken from all research subjects in the morning and tested by a fully-automated electrochemiluminometer to determine the GDNF, CEA and CA199 levels. The sensitivity and specificity of the combined detection of the three indexes for CRC were analyzed, and the ROC curve was plotted to record the area under the curve (AUC). Results. The malignant group had remarkably higher CEA and CA199 levels (P<0.001) and a lower GDNF level (P<0.001) when compared with the benign and control groups. The sensitivity, specificity, positive predictive value and negative predictive value of the combined detection were 96.0%, 94.0%, 88.9% and 97.9%, respectively. The combined detection had the AUC (95% CI) = 0.950 (0.909-0.991), the standard error of 0.021, and the progressive Sig.b<0.001. Conclusion. The combined diagnosis of serum GDNF, CEA and CA199 is a reliable method to improve the diagnostic accuracy of CRC, and this strategy can effectively reduce the missed diagnosis rate and has high application value in clinic.
结直肠癌患者血清GDNF、CEA、CA199的表达水平及诊断价值
背景:探讨胶质细胞系源性神经营养因子(GDNF)、癌胚抗原(CEA)和碳水化合物抗原199 (CA199)在结直肠癌(CRC)患者中的表达水平及诊断价值。& # x0D;方法:选取2020年2月至2021年2月在我院就诊的结直肠癌患者50例为恶性组,结肠良性疾病患者50例为良性组,同期来我院体检的健康人50例为对照组。所有研究对象于早晨空腹外周静脉血,采用全自动电化学发光仪检测GDNF、CEA和CA199水平。分析3项指标联合检测CRC的敏感性和特异性,绘制ROC曲线,记录曲线下面积(AUC)。& # x0D;结果。与良性组和对照组相比,恶性组CEA和CA199水平显著升高(P<0.001), GDNF水平显著降低(P<0.001)。联合检测的敏感性、特异性、阳性预测值和阴性预测值分别为96.0%、94.0%、88.9%和97.9%。联合检测的AUC (95% CI) = 0.950(0.909 ~ 0.991),标准误差为0.021,渐进式Sig.b<0.001。& # x0D;结论。血清GDNF、CEA、CA199联合诊断是提高结直肠癌诊断准确率的可靠方法,该策略可有效降低漏诊率,具有较高的临床应用价值。
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来源期刊
Journal of Medical Biochemistry
Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
3.00
自引率
12.00%
发文量
60
审稿时长
>12 weeks
期刊介绍: The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.
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