Protective Effect of Hedeoma drummondii against Formaldehyde-Induced Testicular Toxicity and Genotoxicity in Wistar Rats

IF 2.1 4区 医学 Q3 ANDROLOGY
Andrologia Pub Date : 2023-10-19 DOI:10.1155/2023/6631345
Nadia Denys Betancourt-Martínez, Pilar Carranza-Rosales, Ezequiel Viveros-Valdez, Nancy Elena Guzmán-Delgado, Maritza Argelia Macias-Corral, Irma Edith Carranza-Torres, Lydia Enith Nava-Rivera, María Soñadora Niño Castañeda, Javier Morán-Martínez
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Abstract

Formaldehyde (FA) is an oxidative stress inducer and a known carcinogen. Hedeoma drummondii has been shown to have potential as a chemopreventive agent. The aim of this study was to assess the protective effect of H. drummondii on FA-induced genotoxic damage, sperm quality parameters, and histological changes of the testis. The male Wistar rats used were divided into the following groups: (G1) 100 mg/kg H. drummondii; (G2) 10 mg/kg FA; (G3) 100 mg/kg H. drummondii + 10 mg/kg FA; (G4) phosphate-buffered saline; (G5) 100 mg/kg α-tocopherol; (G6) 100 mg/kg α-tocopherol + 10 mg/kg FA; and (G7) soybean oil. The results showed a sperm concentration of 23.66 ± 1.52 versus 33.67 cells/mL (p = 0.02), and a percentage of motility of 29.66 ± 1.51 versus 33.33 (p = 0.620), for groups G3 and G6, respectively. Histopathological (H&E) and immunohistochemical analysis of superoxide dismutase showed damage in the germinal epithelium (GE) in G2. While in groups G3 and G6, the histological structure was preserved. A protective effect on sperm DNA fragmentation was also observed for treated groups (G3 and G6, p < 0.0001 vs. G2). While in groups G3 and G6 the histological structure was preserved. A protective effect on sperm DNA fragmentation was also observed for treated groups (G3 and G6). In conclusion, FA exposure significantly reduces sperm quality parameters, causes sperm DNA damage, and alters GE in the testis, while H. drummondii has a protective effect against FA-induced sperm genotoxic damage, similar to α-tocopherol.

Abstract Image

drummondii肿对甲醛致Wistar大鼠睾丸毒性和遗传毒性的保护作用
甲醛(FA)是一种氧化应激诱导剂,也是一种已知的致癌物质。drummondii已被证明是一种潜在的化学预防剂。本研究的目的是评估drummondii对fa诱导的基因毒性损伤、精子质量参数和睾丸组织学变化的保护作用。雄性Wistar大鼠分为以下组:(G1) 100 mg/kg drummondii;(G2) 10 mg/kg FA;(G3) 100 mg/kg drummondii + 10 mg/kg FA;(G4)磷酸盐缓冲盐水;(G5) α-生育酚100 mg/kg;(G6) α-生育酚100 mg/kg + FA 10 mg/kg;(G7)大豆油。结果显示,G3组和G6组的精子浓度分别为23.66±1.52和33.67细胞/mL (p = 0.02),活力百分比分别为29.66±1.51和33.33 (p = 0.620)。组织病理学(H&;E)和超氧化物歧化酶免疫组化分析显示G2的生发上皮(GE)受损。G3、G6组组织结构完整。处理组(G3和G6)也观察到对精子DNA断裂的保护作用,p <;0.0001 vs. G2)。G3、G6组组织结构完整。治疗组(G3和G6)对精子DNA断裂也有保护作用。综上所述,FA暴露显著降低了精子质量参数,造成精子DNA损伤,并改变了睾丸GE,而drummondii对FA诱导的精子遗传毒性损伤具有类似于α-生育酚的保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Andrologia
Andrologia 医学-男科学
CiteScore
5.60
自引率
8.30%
发文量
292
审稿时长
6 months
期刊介绍: Andrologia provides an international forum for original papers on the current clinical, morphological, biochemical, and experimental status of organic male infertility and sexual disorders in men. The articles inform on the whole process of advances in andrology (including the aging male), from fundamental research to therapeutic developments worldwide. First published in 1969 and the first international journal of andrology, it is a well established journal in this expanding area of reproductive medicine.
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