Leaf Morphology Genes SRL1 and RENL1 Co-Regulate Cellulose Synthesis and Affect Rice Drought Tolerance

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science Pub Date : 2024-01-01 DOI:10.1016/j.rsci.2023.10.001

Liu Dan , Zhao Huibo , Wang Zi’an , Xu Jing , Liu Yiting , Wang Jiajia , Chen Minmin , Liu Xiong , Zhang Zhihai , Cen Jiangsu , Zhu Li , Hu Jiang , Ren Deyong , Gao Zhenyu , Dong Guojun , Zhang Qiang , Shen Lan , Li Qing , Qian Qian , Hu Songping , Zhang Guangheng

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Abstract

The morphological development of rice (Oryza sativa L.) leaves is closely related to plant architecture, physiological activities, and resistance. However, it is unclear whether there is a co-regulatory relationship between the morphological development of leaves and adaptation to drought environment. In this study, a drought-sensitive, roll-enhanced, and narrow-leaf mutant (renl1) was induced from a semi-rolled leaf mutant (srl1) by ethyl methane sulfonate (EMS), which was obtained from Nipponbare (NPB) through EMS. Map-based cloning and functional validation showed that RENL1 encodes a cellulose synthase, allelic to NRL1/OsCLSD4. The RENL1 mutation resulted in reduced vascular bundles, vesicular cells, cellulose, and hemicellulose contents in cell walls, diminishing the water-holding capacity of leaves. In addition, the root system of the renl1 mutant was poorly developed and its ability to scavenge reactive oxygen species (ROS) was decreased, leading to an increase in ROS after drought stress. Meanwhile, genetic results showed that RENL1 and SRL1 synergistically regulated cell wall components. Our results revealed a theoretical basis for further elucidating the molecular regulation mechanism of cellulose on rice drought tolerance, and provided a new genetic resource for enhancing the synergistic regulation network of plant type and stress resistance, thereby realizing simultaneous improvement of multiple traits in rice.

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叶片形态基因 SRL1 和 RENL1 共同调控纤维素合成并影响水稻的耐旱性

水稻（Oryza sativa L.）叶片的形态发育与植株的结构、生理活性和抗性密切相关。然而，叶片的形态发育与干旱环境适应之间是否存在共同调控关系尚不清楚。本研究通过甲烷磺酸乙酯（EMS）从半卷叶突变体（strl1）诱导出干旱敏感、卷叶增强和窄叶突变体（renl1）。基于图谱的克隆和功能验证表明，RENL1编码一种纤维素合成酶，与NRL1/OsCLSD4等位。RENL1 突变导致细胞壁中的维管束、泡状细胞、纤维素和半纤维素含量减少，从而降低了叶片的持水能力。此外，renl1 突变体的根系发育不良，清除活性氧（ROS）的能力下降，导致干旱胁迫后 ROS 增加。同时，遗传结果表明，RENL1和SRL1协同调控细胞壁成分。我们的研究结果为进一步阐明纤维素对水稻抗旱性的分子调控机制提供了理论依据，并为增强植株类型与抗逆性的协同调控网络，从而实现水稻多性状的同步改良提供了新的遗传资源。

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来源期刊

Rice Science Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

8.90

自引率

6.20%

发文量

审稿时长

40 weeks

期刊介绍： Rice Science is an international research journal sponsored by China National Rice Research Institute. It publishes original research papers, review articles, as well as short communications on all aspects of rice sciences in English language. Some of the topics that may be included in each issue are: breeding and genetics, biotechnology, germplasm resources, crop management, pest management, physiology, soil and fertilizer management, ecology, cereal chemistry and post-harvest processing.