Assessment of plasma melatonin profile in summer anestrous buffaloes exhibiting differential fertility following melatonin implants treatment

IF 0.2 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Buffalo Bulletin Pub Date : 2023-10-04 DOI:10.56825/bufbu.2023.4233571

Sarvpreet Singh Ghuman, Mrignak Honparkhe, Bilawal Singh

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引用次数: 0

Abstract

Plasma melatonin vis-a-vis anestrus or estrus status of buffaloes during summer or winter season (n=50 in each group) suggested its impact on reproductive axis as plasma melatonin activity tended to be higher (P>0.05) in buffaloes exhibiting estrus compared to their anestrous counterparts irrespective of season. The differential reproductive status of these buffaloes was confirmed by history as well as differences (P<0.05) in diameter of largest follicle and luteal profile. Further, for evaluating the impact of melatonin treatment during summer season on reproductive axis, 132 anestrous buffaloes were subcutaneously inserted 2x4 mm absorbable slow-release melatonin implants (18 mg/50 kg b wt) at the base of left ear and 60 buffaloes were used as control. In these buffaloes, ovarian ultrasonography and jugular vein blood sampling was carried out at 7-day interval till day 35 post-treatment or till ovulation, whichever was earlier. Control and implanted buffaloes were subjected to artificial insemination (AI) at overt or induced estrus followed by pregnancy diagnosis at day 90 post-AI. In treatment group, an increase (P<0.05) in plasma melatonin was recorded in all the buffaloes, exhibiting differential fertility status, during post-treatment study period compared to their pre-treatment and control group values. However, within treatment group, there was no difference (P>0.05) in plasma melatonin between ovulatory or non-ovulatory as well as between pregnant or non-pregnant counterparts. Moreover, plasma melatonin within control buffaloes remained similar (P>0.05) throughout the study period irrespective of differential exhibition of fertility. It can be concluded that factors other than circulating melatonin are also involved in the display of differential fertility in terms of initiation of ovarian cyclicity/ovulation or ability to conceive in summer anestrous buffaloes.

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褪黑素植入治疗后表现出不同生育能力的夏季失情水牛血浆褪黑素谱的评估

血浆褪黑素与夏季或冬季水牛的发情或发情状态的关系(每组n=50)表明其对生殖轴的影响，因为无论季节如何，处于发情状态的水牛血浆褪黑素活性往往高于处于无情状态的水牛(P>0.05)。历史以及最大卵泡直径和黄体轮廓的差异(P<0.05)证实了这些水牛的不同生殖状态。此外，为了评估夏季褪黑素治疗对生殖轴的影响，我们在132头发情水牛的左耳基部皮下植入2x4 mm可吸收的缓释褪黑素植入物(18 mg/50 kg b wt)，并以60头水牛作为对照。每隔7天进行卵巢超声检查和颈静脉采血，直至治疗后第35天或排卵前(以较早者为准)。对照和植入水牛在明显或诱导发情时进行人工授精，并在人工授精后第90天进行妊娠诊断。在治疗组，与治疗前和对照组相比，在治疗后的研究期间，所有水牛血浆褪黑素均增加(P<0.05)，表现出不同的生育状态。而在治疗组内，排卵期与非排卵期、孕妇与非孕妇血浆褪黑素水平差异无统计学意义(P>0.05)。此外，在整个研究期间，对照水牛的血浆褪黑素保持相似(P>0.05)，而不考虑生育表现的差异。由此可以得出结论，除循环褪黑素外，其他因素也参与了在卵巢周期/排卵启动或夏季无情水牛受孕能力方面表现出的差异生育能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Buffalo Bulletin AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

0.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Buffalo Bulletin is published quarterly in January-March, April-June, July-September and October-December. Contributions on any aspect of research or development, progress reports of projects and news on buffalo will be considered for publication in the bulletin.