Insecticide Resistance Development and Detoxification Enzyme Activities of Spodoptera litura (Fabricius) in Soybean from Kumaon Himalayas

IF 0.8 4区生物学 Q3 AGRONOMY

Legume Research Pub Date : 2023-05-30 DOI:10.18805/lr-5101

Rashmi Joshi, Neeta Gaur, Sudha Mathpal

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引用次数: 0

Abstract

Background: Soybean is an important crop of Uttarakhand both in hills as well as in plains and Spodoptera litura is an important pest of soybean in Uttarakhand. Several insecticides have been in use for its management and resistance has been observed against different group of insecticides. Thus, to study the level of infestation and insecticide resistance from soybean fields of hill farmers of Kumaon Himalaya, survey and experiments were conducted. Methods: Survey was conducted and scale of infestation was determined. Chemical and biochemical assay were conducted to study insecticide resistance. For chemical assay IRAC Method No. 7 using insecticides indoxacarb 14.5% SC, chlorantraniliprole 18.5% Sc, fipronil 5% SC, chlorpyriphos 50%+cypermethrin 5% EC and profenofos 40%+cypermethrin 4% EC and for biochemical assay the specific activity of Carboxylesterase, Acetyl choline esterase, Mono-oxygenase and Glutathione-S-transferase was studied. Result: The infestation level from different regions showed the presence of S. litura, ranging from mild to severe infestation. The resistance ratio of different insecticides used ranged from a low to an extremely high resistance level with LC50 ranging from 0.37 µg/ml to 1648.84 µg/ml. The biochemical analysis of carboxylesterase, acetylcholinesterase, monooxygenase and gluthione-s-transferase showed an overproduction of these detoxification enzymes in the resistant population.

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Kumaon - Himalayas大豆斜纹夜蛾(Spodoptera斜纹夜蛾)抗性发育及解毒酶活性

背景:大豆是北阿坎德邦丘陵和平原的重要作物，斜纹夜蛾是北阿坎德邦重要的大豆害虫。已使用几种杀虫剂对其进行管理，并观察到对不同种类杀虫剂的抗药性。为此，通过调查和试验，研究了库马高原山地农民大豆田的侵染程度和对杀虫剂的抗性。方法:进行调查，确定侵害规模。采用化学生化试验研究其抗药性。化学分析采用IRAC法7号，采用茚虫威14.5% SC、氯虫腈18.5% SC、氟虫腈5% SC、毒死蜱50%+高效氯氰菊酯5% EC、丙烯磷40%+高效氯氰菊酯4% EC进行，生化分析采用羧酯酶、乙酰胆碱酯酶、单加氧酶、谷胱甘肽s -转移酶的比活性。结果:不同地区均有斜纹夜蛾侵染，侵染程度由轻到重。不同杀虫剂的抗性比从低到极高，LC50为0.37µg/ml ~ 1648.84µg/ml。羧酸酯酶、乙酰胆碱酯酶、单加氧酶和谷胱甘肽-s转移酶的生化分析表明，抗性群体中这些解毒酶过量产生。

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来源期刊

Legume Research AGRONOMY-

CiteScore

1.30

自引率

37.50%

发文量

256

审稿时长

6-12 weeks

期刊介绍： Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.