{"title":"Optimizing Priming Concentration and Duration of PEG 6000 for Improving Seed Germination and Vigour in Chickpea (Cicer arietinum L.)","authors":"Anish Choudhury, Sanjoy Kumar Bordolui, Jui Ray","doi":"10.60151/envec/wykc4262","DOIUrl":null,"url":null,"abstract":"This study was undertaken with different concentration and duration of PEG-6000 to observe the effect of germination and seedling vigour in chickpea seeds. The experiment was conducted in seed testing laboratory, Department of Seed Science and Technology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, India during 2020-2021 following Complete Randomized Block Design with three replications in order to standardize the appropriate concentration and duration while soaking the seeds within PEG-6000 solution for screening a range of duration concentrations viz, 10 g liter-1 for 6 hrs, 10 g liter-1 for 8 hrs, 10 g liter-1 for 10 hrs, 15 g liter-1 for 6 hrs , 15 g liter-1 for 8 hrs, 15 g liter-1 for 10 hrs, 20 g liter-1 for 6 hrs, 20 g liter-1 for 8 hrs, 20 g liter-1 for 10 hrs, 25 g liter-1 for 6 hrs, 25 g liter-1 for 8 hrs, 25 g liter-1 for 10 hrs. Untreated dry seeds were considered as control. It was found that all the priming treatments showed significance difference in overall including the control. The best performer or the better result was shown by T8 which is 20 gm/ liter solution with the soaking duration of 8 hrs. It was highest performer in germination percentage (93.77), root length (6.57 cm), shoot length (16.57 cm), seedling fresh weight (1.66 g), seedling dry weight (0.14 g), seedling vigour index I (2137.07), time to 50% germination (2.33 days) and germination index (77.82). This study showed that seed priming with osmotic solution could improve some seedling parameters in chickpea. In seed priming, its simplicity such as no requirements for extensive equipment and chemicals could be used method for overcoming problems related to a poor germination and further seedling establishment and helps in sustaining agriculture with a cost effective approach and with the help of seed priming treatments which are non-toxic and from eco-friendly sources.","PeriodicalId":36141,"journal":{"name":"Environment and Ecology Research","volume":"386 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environment and Ecology Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.60151/envec/wykc4262","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Environmental Science","Score":null,"Total":0}
引用次数: 0
Abstract
This study was undertaken with different concentration and duration of PEG-6000 to observe the effect of germination and seedling vigour in chickpea seeds. The experiment was conducted in seed testing laboratory, Department of Seed Science and Technology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, India during 2020-2021 following Complete Randomized Block Design with three replications in order to standardize the appropriate concentration and duration while soaking the seeds within PEG-6000 solution for screening a range of duration concentrations viz, 10 g liter-1 for 6 hrs, 10 g liter-1 for 8 hrs, 10 g liter-1 for 10 hrs, 15 g liter-1 for 6 hrs , 15 g liter-1 for 8 hrs, 15 g liter-1 for 10 hrs, 20 g liter-1 for 6 hrs, 20 g liter-1 for 8 hrs, 20 g liter-1 for 10 hrs, 25 g liter-1 for 6 hrs, 25 g liter-1 for 8 hrs, 25 g liter-1 for 10 hrs. Untreated dry seeds were considered as control. It was found that all the priming treatments showed significance difference in overall including the control. The best performer or the better result was shown by T8 which is 20 gm/ liter solution with the soaking duration of 8 hrs. It was highest performer in germination percentage (93.77), root length (6.57 cm), shoot length (16.57 cm), seedling fresh weight (1.66 g), seedling dry weight (0.14 g), seedling vigour index I (2137.07), time to 50% germination (2.33 days) and germination index (77.82). This study showed that seed priming with osmotic solution could improve some seedling parameters in chickpea. In seed priming, its simplicity such as no requirements for extensive equipment and chemicals could be used method for overcoming problems related to a poor germination and further seedling establishment and helps in sustaining agriculture with a cost effective approach and with the help of seed priming treatments which are non-toxic and from eco-friendly sources.
采用不同浓度、不同处理时间的PEG-6000对鹰嘴豆种子萌发和幼苗活力的影响进行了研究。该实验于2020-2021年在印度西孟加拉邦纳迪亚莫汉普尔Bidhan Chandra Krishi Viswavidyalaya种子科学与技术部种子测试实验室进行,遵循完全随机区组设计,进行3次重复,以标准化适当的浓度和持续时间,同时将种子浸泡在PEG-6000溶液中筛选持续时间浓度范围,即10 g l -1浸泡6小时,10 g l -1浸泡8小时,10小时10克升1,6小时15克升1,8小时15克升1,10小时15克升1,6小时20克升1,8小时20克升1,10小时20克升1,6小时25克升1,8小时25克升1,10小时25克升1。以未处理的干种子为对照。结果表明,包括对照在内,各启动处理在总体上有显著性差异。T8浸泡时间为8小时,溶液浓度为20 gm/ l,效果最好。在发芽率(93.77)、根长(6.57 cm)、茎长(16.57 cm)、幼苗鲜重(1.66 g)、干重(0.14 g)、幼苗活力指数I(2137.07)、萌发至50%时间(2.33 d)和萌发指数(77.82)方面表现最好。本研究表明,渗透液灌种可以改善鹰嘴豆幼苗的一些参数。在种子催种方面,它的简单性,如不需要大量的设备和化学品,可以用来克服与发芽率低和进一步建立幼苗有关的问题,并有助于以成本效益的方法维持农业,并帮助种子催种处理,这是无毒的和生态友好的来源。