Determination of the Effect of Epigallocatechin Gallate on Oxidative Stress, Apoptosis and Sperm Quality in Rabbit Semen

New Trends in Medicine Sciences Pub Date : 2023-09-27 DOI:10.56766/ntms.1340936

Serkan Ali AKARSU, Recep Hakkı KOCA, Aslıhan ÇAKIR CİHANGİROĞLU, Tutku Can ACISU, İbrahim Halil GÜNGÖR, Mustafa SÖNMEZ, Gaffari TÜRK, Seyfettin GÜR, Ali Doğan ÖMÜR, Harika Eylül ESMER DURUEL

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Abstract

Objectives;In this study, it was aimed to determine the effect of Epigallocatechin gallate (EGCG) on the cryopreservation of rabbit semen. Material and metod; For this purpose, semen was collected from six male New Zealand rabbits. Collected semen were divided into 5 groups as control, 25 µM, 50 µM, 100 µM and 200 µM. Semen samples were reconstituted and frozen after adding EGCG. Frozen straws were then thawed. Results; After freezing and thawing, total motility, progressive motility, and rate of fast and medium speed sperm were significantly higher in the 50 µM group than in the other groups. Static sperm ratio was found to be lower in the 50 µM group than in the other groups. When the flow cytometry results were examined, there was no statistical difference between the groups in terms of the ratio of dead and live sperm. However, the rate of dead sperm with acrosome damage was found to be the lowest in the 50 µM group. High mitochondrial membrane potential sperm ratio was found higher in 50 µM and 100 µM groups compared to other groups (p

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表没食子儿茶素没食子酸酯对兔精液氧化应激、细胞凋亡及精子质量影响的测定

目的:研究没食子儿茶素没食子酸酯(EGCG)对兔精液冷冻保存的影响。& # x0D;材料及方法;为此，采集了6只雄性新西兰兔的精液。采集的精液分为对照组、25µM组、50µM组、100µM组和200µM组。精液样品加入EGCG后重组冷冻。然后解冻冷冻的稻草。& # x0D;结果;冷冻和解冻后，50µM组精子总活力、渐进活力、快、中速精子率均显著高于其他各组。静态精子比例在50µM组低于其他各组。流式细胞术检测结果显示，两组之间活精子与死精子的比例差异无统计学意义。然而，顶体损伤的死精子率在50µM组中最低。50µM和100µM组高线粒体膜电位精子比高于其他组(p

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New Trends in Medicine Sciences

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