Exploration of the Potential of Bacillus spp. as an Antagonist and PGPR against Stem and Pod Rot of Groundnut.

IF 0.8 4区生物学 Q3 AGRONOMY

Legume Research Pub Date : 2023-10-20 DOI:10.18805/lr-5100

Gururaj Sunkad, Khadarbi ., Meghana S. Patil, Ranjana Joshi

{"title":"Exploration of the Potential of Bacillus spp. as an Antagonist and PGPR against Stem and Pod Rot of Groundnut.","authors":"Gururaj Sunkad, Khadarbi ., Meghana S. Patil, Ranjana Joshi","doi":"10.18805/lr-5100","DOIUrl":null,"url":null,"abstract":"Background: Stem and pod rot is an economically important soil borne disease of groundnut caused by Sclerotium rolfsii Sacc. resulting in significant yield losses across the globe. Bacillus spp. is rod-shaped and gram-positive bacterium which is predominantly found in the soil. Hence, considering the importance of the disease, the present investigation was carried out to evaluate the indigenous Bacillus spp. for plant growth promotion and to inhibit the pathogen in groundnut. Methods: Totally nineteen Bacillus spp. isolates from healthy groundnut rhizosphere and their antagonistic potential was recorded. The molecular characterization of five efficient Bacillus spp. was carried out using 16S rDNA. Later, morphological and biochemical characterization and plant growth promoting traits were recorded. Result: All nineteen isolates of Bacillus spp. showed antagonistic potential against S. rolfsii. Five Bacillus spp. isolates which showed higher inhibition of the pathogen were identified as B. subtilis, B. pacific, B. cereus, two isolates belonging to B. tropicus through molecular technique. The morphological, biochemical characters and growth promoting traits were recorded.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"19 1 1","pages":"0"},"PeriodicalIF":0.8000,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Legume Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18805/lr-5100","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Stem and pod rot is an economically important soil borne disease of groundnut caused by Sclerotium rolfsii Sacc. resulting in significant yield losses across the globe. Bacillus spp. is rod-shaped and gram-positive bacterium which is predominantly found in the soil. Hence, considering the importance of the disease, the present investigation was carried out to evaluate the indigenous Bacillus spp. for plant growth promotion and to inhibit the pathogen in groundnut. Methods: Totally nineteen Bacillus spp. isolates from healthy groundnut rhizosphere and their antagonistic potential was recorded. The molecular characterization of five efficient Bacillus spp. was carried out using 16S rDNA. Later, morphological and biochemical characterization and plant growth promoting traits were recorded. Result: All nineteen isolates of Bacillus spp. showed antagonistic potential against S. rolfsii. Five Bacillus spp. isolates which showed higher inhibition of the pathogen were identified as B. subtilis, B. pacific, B. cereus, two isolates belonging to B. tropicus through molecular technique. The morphological, biochemical characters and growth promoting traits were recorded.

查看原文本刊更多论文

芽孢杆菌作为花生茎腐病拮抗剂和PGPR潜力的探索。

背景:花生茎、荚果腐病是由罗氏菌核菌引起的一种重要的土传病害。导致全球范围内的重大产量损失。芽孢杆菌是一种杆状革兰氏阳性细菌，主要存在于土壤中。因此，考虑到该病害的重要性，本研究旨在评价本地芽孢杆菌对花生植株生长的促进作用和对病原菌的抑制作用。方法:对健康花生根际分离的19株芽孢杆菌进行拮抗活性测定。利用16S rDNA对5株高效芽孢杆菌进行了分子鉴定。随后，对其形态生化特性和促进植株生长的性状进行记录。结果:19株芽孢杆菌均对罗氏葡萄球菌有拮抗作用。通过分子技术鉴定出对病原菌有较强抑制作用的5株芽孢杆菌分别为枯草芽孢杆菌、太平洋芽孢杆菌、蜡样芽孢杆菌，2株属热带芽孢杆菌。记录了其形态、生化特性和促生长性状。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Legume Research AGRONOMY-

CiteScore

1.30

自引率

37.50%

发文量

256

审稿时长

6-12 weeks

期刊介绍： Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.