José R. Pérez-Calderón, Jorge A. Pérez-León, Nuno Simões, Marisela Aguirre-Ramírez, Roxana E. Malpica-Calderón, Alejandro Botello
{"title":"Extravisual opsins in the blind shrimp Creaseria morleyi: presence and expression","authors":"José R. Pérez-Calderón, Jorge A. Pérez-León, Nuno Simões, Marisela Aguirre-Ramírez, Roxana E. Malpica-Calderón, Alejandro Botello","doi":"10.1590/2358-2936e2023024","DOIUrl":null,"url":null,"abstract":"The presence of a long-wavelength sensitive (LWS) opsin gene was demonstrated in the stygobitic crustacean Creaseria morleyi (Creaser, 1936) by PCR readings from genomic DNA. In order to find the expression of this gene in extraocular tissue, shrimps were collected and placed in a tank to expose them to light/dark conditions for a period of 72 hours, and immediately after, sacrificed and sampled for RNA in the eyes, cephalothorax, abdomen, and sixth abdominal segment plus uropods. The transcripts of the LWS opsin gene were found in the eyes and abdomen of individuals exposed to light. The expression of these opsins could be involved in extravisual functions such as synchronization of their biological processes with environmental cycles related to diurnal vertical migration.","PeriodicalId":56265,"journal":{"name":"Nauplius","volume":"45 1","pages":"0"},"PeriodicalIF":0.8000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nauplius","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/2358-2936e2023024","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The presence of a long-wavelength sensitive (LWS) opsin gene was demonstrated in the stygobitic crustacean Creaseria morleyi (Creaser, 1936) by PCR readings from genomic DNA. In order to find the expression of this gene in extraocular tissue, shrimps were collected and placed in a tank to expose them to light/dark conditions for a period of 72 hours, and immediately after, sacrificed and sampled for RNA in the eyes, cephalothorax, abdomen, and sixth abdominal segment plus uropods. The transcripts of the LWS opsin gene were found in the eyes and abdomen of individuals exposed to light. The expression of these opsins could be involved in extravisual functions such as synchronization of their biological processes with environmental cycles related to diurnal vertical migration.