Extravisual opsins in the blind shrimp Creaseria morleyi: presence and expression

IF 0.8 4区 生物学 Q4 MARINE & FRESHWATER BIOLOGY
Nauplius Pub Date : 2023-01-01 DOI:10.1590/2358-2936e2023024
José R. Pérez-Calderón, Jorge A. Pérez-León, Nuno Simões, Marisela Aguirre-Ramírez, Roxana E. Malpica-Calderón, Alejandro Botello
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引用次数: 0

Abstract

The presence of a long-wavelength sensitive (LWS) opsin gene was demonstrated in the stygobitic crustacean Creaseria morleyi (Creaser, 1936) by PCR readings from genomic DNA. In order to find the expression of this gene in extraocular tissue, shrimps were collected and placed in a tank to expose them to light/dark conditions for a period of 72 hours, and immediately after, sacrificed and sampled for RNA in the eyes, cephalothorax, abdomen, and sixth abdominal segment plus uropods. The transcripts of the LWS opsin gene were found in the eyes and abdomen of individuals exposed to light. The expression of these opsins could be involved in extravisual functions such as synchronization of their biological processes with environmental cycles related to diurnal vertical migration.
盲虾morleycreaseria视外蛋白的存在与表达
通过基因组DNA的PCR读数,证明了粘生甲壳类Creaseria morleyi (Creaser, 1936)中存在一个长波敏感(LWS)视蛋白基因。为了寻找该基因在眼外组织中的表达,收集虾并将其置于水箱中,在光照/黑暗条件下暴露72小时,然后立即处死并在眼睛,头胸,腹部,第六腹段和尾足中取样RNA。在暴露于光的个体的眼睛和腹部发现了LWS视蛋白基因的转录本。这些视蛋白的表达可能涉及外视功能,如与昼夜垂直迁移相关的环境循环同步的生物过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nauplius
Nauplius Multiple-
自引率
14.30%
发文量
22
审稿时长
24 weeks
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